problendoПроблемы ЭндокринологииProblems of Endocrinology0375-96602308-1430Endocrinology Research Centre10.14341/probl10140problendo-10140Research ArticleОбзорыReviewsПрименение конфокальной микроскопии в фундаментальных исследованиях и клинической практике эндокринолога: современные возможностиThe use of confocal microscopy in experimental studies and clinical practice of an endocrinologist: modern opportunitieshttps://orcid.org/0000-0002-9717-9742МокрышеваНаталья ГеоргиевнаMokryshevaNatalya G.

д.м.н., профессор

MD, PhD, Professor

nm70@mail.ruhttps://orcid.org/0000-0001-7921-6987КиселевСергей ЛьвовичKiselevSergey L.

д.б.н., профессор

PhD, Professor

sl_kiselev@yahoo.comhttps://orcid.org/0000-0002-2742-372XКлементьеваНаталия ВладимировнаKlementievaNatalia V.

к.б.н.

PhD

nvklementieva@gmail.comhttps://orcid.org/0000-0003-2669-9457ГорбачеваАнна МаксимовнаGorbachevaAnna M.

MD

ann.gorbachewa@yandex.ruhttps://orcid.org/0000-0002-8175-7886ДедовИван ИвановичDedovIvan I.

д.м.н., профессор, академик РАН

MD, PhD, Professor

dedov@endocrincentr.ru<p>Национальный медицинский исследовательский центр эндокринологии</p>Россия<p>Endocrinology research centre</p>Russian Federation<p class="Text05">Институт общей генетики им. Н.И. Вавилова Российской академии наук</p>Россия<p>Vavilov Institute of General Genetics of the Russian Academy of Sciences</p>Russian Federation<p>Приволжский исследовательский медицинский университет</p>Россия<p>Privolzhskiy Research Medical University</p>Russian Federation<p>Национальный медицинский исследовательский центр эндокринологии</p>Россия<p>Endocrinology Research Centre</p>Russian Federation<p>Национальный медицинский исследовательский центр эндокринологии; Первый Московский государственный медицинский университет им. И.М. Сеченова</p>Россия<p>Endocrinology Research Centre; I.M. Sechenov First Moscow State Medical University</p>Russian Federation201912092019653174183Copyright &#x00A9; Мокрышева Н.Г., Киселев С.Л., Клементьева Н.В., Горбачева А.М., Дедов И.И., 20192019Мокрышева Н.Г., Киселев С.Л., Клементьева Н.В., Горбачева А.М., Дедов И.И.Mokrysheva N.G., Kiselev S.L., Klementieva N.V., Gorbacheva A.M., Dedov I.I.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.probl-endojournals.ru/jour/article/view/10140

Конфокальная микроскопия – современный метод визуализации, открывающий широкие возможности для исследований in vitro и in vivo. В клинической части обзора основное внимание уделено прочно вошедшим в практику методикам, таким как конфокальная микроскопия роговицы при диагностике диабетической нейропатии или эндокринной офтальмопатии; кратко описаны новые методики (интраоперационная оценка тканей, получаемых при удалении аденом гипофиза, щитовидной и околощитовидной желез). В части, посвященной фундаментальным исследованиям, рассматривается использование конфокальной микроскопии для характеристики колокализации белков, а также трехмерных внутриклеточных структур и сигнальных путей in vivo. Анализируются индикаторы внутриклеточного кальция.

Confocal microscopy is a modern imaging method that provides ample opportunities for in vitro and in vivo research. The clinical part of the review focuses on well-established techniques, such as corneal confocal microscopy for the diagnosis of diabetic neuropathy or endocrine ophthalmopathy; new methods are briefly described (intraoperative evaluation of tissues obtained by removing pituitary adenomas, thyroid and parathyroid glands). In the part devoted to fundamental research, the use of confocal microscopy to characterize the colocalization of proteins, as well as three-dimensional intracellular structures and signaling pathways in vivo, is considered. Indicators of intracellular calcium are analyzed.

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The authors declare that there are no conflicts of interest present.