problendoПроблемы ЭндокринологииProblems of Endocrinology0375-96602308-1430Endocrinology Research Centre10.14341/probl12770problendo-12770Research ArticleЭкспериментальная эндокринологияExperimental endocrinologyПролактолиберин увеличивает тревожность крысProlactin-releasing peptide increases rat anxietyhttps://orcid.org/0000-0002-5716-0027ЖуковД. А.ZhukovD. A.

Жуков Дмитрий Анатольевич – доктор биологических наук, доцент, старший научный сотрудник лаборатории сравнительной генетики поведения.

199034, Санкт-Петербург, наб. ­Макарова, д. 6.

eLibrary SPIN: 1416-0551

Dmitry A. Zhukov – PhD.

6 Makarova, 199034, St-Petersburg.

eLibrary SPIN: 1416-0551

dazhukov0@gmail.comhttps://orcid.org/0000-0002-2867-044XМарковА. Г.MarkovA. G.

Марков Александр Георгиевич - доктор биологических наук, профессор.

Санкт-Петербург.

eLibrary SPIN: 4985-0808

Alexander G. Markov – PhD.

St-Petersburg.

eLibrary SPIN: 4985-0808

a.markov@spbu.ruhttps://orcid.org/0000-0003-2275-4084ВиноградоваЕ. П.VinogradovaE. P.

Виноградова Екатерина Павловна – кандидат биологических наук, доцент.

Санкт-Петербург.

eLibrary SPIN: 4899-1537

Ekaterina P. Vinogradova – PhD.

St-Petersburg.

eLibrary SPIN: 4899-1537

e.vinogradova@spbu.ruИнститут физиологии им. И.П. Павлова РАНРоссияPavlov Institute of Physiology RASRussian FederationБиологический факультет СПбГУРоссияBiological Faculty, SPb State UniversityRussian Federation2021190920216752933Copyright © Жуков Д.А., Марков А.Г., Виноградова Е.П., 20212021Жуков Д.А., Марков А.Г., Виноградова Е.П.Zhukov D.A., Markov A.G., Vinogradova E.P.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.probl-endojournals.ru/jour/article/view/12770ОБОСНОВАНИЕ

ОБОСНОВАНИЕ. Пролактолиберин, или пролактин-рилизинг-гормон (Прл-РГ), помимо стимуляции выработки пролактина, взаимодействует с различными отделами ЦНС, участвуя в реализации многих функций, отражающихся в поведении.

ЦЕЛЬ

ЦЕЛЬ. Поскольку в литературе нет данных о связи Прл-РГ с тревожностью, целью данной работы явилось изучение влияния Прл-РГ на тревожность белых крыс Вистар.

МАТЕРИАЛЫ И МЕТОДЫ

МАТЕРИАЛЫ И МЕТОДЫ. Тревожность оценивали в двух тестах. В приподнятом крестообразном лабиринте регистрировали время, проводимое в открытых рукавах и количество реакций свешивания, В тесте социального предпочтения регистрировали время, проводимое возле чужака, возле знакомой особи и на нейтральной территории.

РЕЗУЛЬТАТЫ

РЕЗУЛЬТАТЫ. Введение Прл-РГ в дозе 10-10 М объемом 10 мкл в каждую ноздрю уменьшало время, проводимое животными в открытых рукавах приподнятого крестообразного лабиринта, и количество реакций свешивания. Для тестирования социального взаимодействия животных предварительно отбирали по высокому либо низкому уровням тревожности в тесте с приподнятым крестообразным лабиринтом. У крыс с изначально низким уровнем тревожности Прл-РГ уменьшал время, проводимое рядом с незнакомцем, что указывает на повышение уровня тревожности. Поведение крыс с изначально высоким уровнем тревоги не менялось после введения Прл-РГ.

ЗАКЛЮЧЕНИЕ

ЗАКЛЮЧЕНИЕ. Результаты наших экспериментов свидетельствуют о том, что интраназальное введение Прл-РГ увеличивает тревожность крыс.

BACKGROUND

BACKGROUND: Prolactin-releasing peptide(Prl-RP), in addition to stimulating the production of prolactin, interacts with various parts of the central nervous system, participating in the implementation of many functions that are reflected in behavior.

AIM

AIM: The effect of Prl-RP on the anxiety of white Wistar rats was studied since there were no data in the literature on the relationship between Prl-RP and anxiety.

MATERIALS AND METHODS

MATERIALS AND METHODS: Anxiety was assessed in two tests. In the elevated plus-maze (EPM), the time spent in the open arms and the number of edge reactions were recorded. In the social preference test, the time spent near a stranger, near a familiar individual, and in neutral territory were recorded.

RESULTS

RESULTS: The administration of Prl-RP at a dose of 10-10 M with a volume of 10 µl in each nostril reduced the time spent by the animals in the open arms of the EPM, and the number of edge reactions. For testing the social interaction, animals were pre-selected for high or low levels of anxiety in the EPM. In rats with initially low levels of anxiety, Prl-RP reduced the time spent near a stranger, indicating an increase in anxiety levels. The behavior of rats with initially high levels of anxiety did not change after application of the Prl-RP.

CONCLUSION

CONCLUSION: The results of our experiments indicate that the intranasal administration of Prl-RP increases the anxiety of rats.

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The authors declare that there are no conflicts of interest present.