problendoПроблемы ЭндокринологииProblems of Endocrinology0375-96602308-1430Endocrinology Research Centre10.14341/probl12817problendo-12817Research ArticleКлиническая эндокринологияClinical endocrinologyЭкспрессии микроРНК в плазме крови, оттекающей от гипофиза, у пациентов с болезнью Иценко-Кушинга и АКТГ-эктопированным синдромомDifferences in plasma miRNA levels in inferior petrosal sinus samples of patients with ACTH-dependent Cushing’s syndromehttps://orcid.org/0000-0002-4567-2412МалыгинаА. А.MalyginaA. A.

Малыгина Анастасия Андреевна

117036, Москва, ул. Дм. Ульянова, д. 11

eLibrary SPIN: 8990-8260

Anastasia A. Malygina, MD

11 Dm. Ulyanova street, 117036 Moscow

eLibrary SPIN: 8990-8260

malygina.aa@gmail.comhttps://orcid.org/0000-0002-6674-6441БелаяЖ. Е.BelayaZ. E.

Белая Жанна Евгеньевна, доктор медицинских наук

Москва

eLibrary SPIN: 4746-7173

Zhanna E. Belaya, MD, PhD

Moscow

eLibrary SPIN: 4746-7173

jannabelaya@gmail.comhttps://orcid.org/0000-0001-9762-3383НикитинА. Г.NikitinA. G.

Никитин Алексей Георгиевич, кандидат биологических наук

Москва

eLibrary SPIN: 3367-0680

 

Alexey G. Nikitin, PhD

Moscow

eLibrary SPIN: 3367-0680

avialn@gmail.comhttps://orcid.org/0000-0001-9512-9277КошкинФ. А.KoshkinP. A.

Кошкин Филипп Александрович, кандидат биологических наук

Москва

eLibrary SPIN: 5627-2121

Philipp A. Koshkin, PhD

Moscow

eLibrary SPIN: 5627-2121

philipkoshkin@gmail.comhttps://orcid.org/0000-0002-2175-3170СиткинИ. И.SitkinI. I.

Ситкин Иван Иванович, кандидат медицинских наук

Москва

eLibrary SPIN: 9779-3780

Ivan I. Sitkin, MD, PhD

Moscow

eLibrary SPIN: 9779-3780

sitkin_ivan@rambler.ruhttps://orcid.org/0000-0003-4353-6705ЛапшинаА. М.LapshinaA. M.

Лапшина Анастасия Михайловна, кандидат медицинских наук

Москва

eLibrary SPIN:1582-5033

Anastasia M. Lapshina, MD, PhD

Moscow

eLibrary SPIN:1582-5033

nottoforget@yandex.ruhttps://orcid.org/0000-0002-6993-5096ХандаеваП. М.KhandaevaP. M.

Хандаева Патимат Магомедовна

Москва

eLibrary SPIN: 6950-5200

Patimat M. Khandaeva, MD

Moscow

eLibrary SPIN: 6950-5200

pati_khandaeva@mail.ruhttps://orcid.org/0000-0002-9314-7831ЛуценкоА. С.LutsenkoA. S.

Луценко Александр Сергеевич

Москва

eLibrary SPIN: 4037-1030

Alexander S. Lutsenko, MD

Moscow

eLibrary SPIN: 4037-1030

some91@mail.ruhttps://orcid.org/0000-0002-1359-8297ТрухинаД. А.TrukhinaD. A.

Трухина Диана Аршалуйсовна, клинический ординатор

Москва

elibrary SPIN: 5618-8971

Diana A. Trukhina, MD

Moscow

elibrary SPIN: 5618-8971

diadavtyan@gmail.comhttps://orcid.org/0000-0002-5634-7877МельниченкоГ. А.MelnichenkoG. A.

Мельниченко Галина Афанасьевна, доктор медицинских наук, профессор, академик РАН

Москва

eLibrary SPIN: 8615-0038

Galina A. Melnichenko, MD, PhD, professor

Moscow

eLibrary SPIN: 8615-0038

teofrast2000@mail.ruНациональный медицинский исследовательский центр эндокринологииРоссияThe National Medical Research Center for EndocrinologyRussian FederationНаучно-исследовательский институт пульмонологииРоссияPulmonology Scientific Research Institute under FMBA of RussiaRussian FederationМедико-генетический центр «Геномед»РоссияCenter of medical genetics «Genomed»Russian Federation2021301220216761830Copyright © Малыгина А.А., Белая Ж.Е., Никитин А.Г., Кошкин Ф.А., Ситкин И.И., Лапшина А.М., Хандаева П.М., Луценко А.С., Трухина Д.А., Мельниченко Г.А., 20212021Малыгина А.А., Белая Ж.Е., Никитин А.Г., Кошкин Ф.А., Ситкин И.И., Лапшина А.М., Хандаева П.М., Луценко А.С., Трухина Д.А., Мельниченко Г.А.Malygina A.A., Belaya Z.E., Nikitin A.G., Koshkin P.A., Sitkin I.I., Lapshina A.M., Khandaeva P.M., Lutsenko A.S., Trukhina D.A., Melnichenko G.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.probl-endojournals.ru/jour/article/view/12817ОБОСНОВАНИЕ

ОБОСНОВАНИЕ.  За последние десятилетия микроРНК зарекомендовали себя как новые маркеры для целого ряда заболеваний. Определение специфичной панели микроРНК, отличающей пациентов с БИК и АКТГ-эктопированным синдромом (АКТГ-ЭС), смогло бы значительно упростить диагностику.

ЦЕЛЬ

ЦЕЛЬ. Выявить циркулирующие микроРНК, отличающиеся у пациентов с БИК и АКТГ-ЭС в плазме крови, оттекающей от гипофиза.

МАТЕРИАЛЫ И МЕТОДЫ

МАТЕРИАЛЫ И МЕТОДЫ. Выполнено одноцентровое, одномоментное, выборочное исследование случай-контроль. Включено 24 пациента с АКТГ-зависимым ЭГ, которым требовалось проведение селективного забора крови из нижних каменистых синусов (НКС). Из них 12 пациентов с БИК (м=2, ж=10), возрастная медиана 46,5 лет [33,8;53,5] и 12 пациентов с АКТГ-ЭС (м=4, ж=8), возрастная медиана 54 года [38,75;60,75].  Образцы плазмы крови из НКС были получены до введения стимуляционного агента, центрифугированы и заморожены при температуре -80С. Выделение микроРНК из плазмы крови проводили с помощью miRNeasy Serum/Plasma Kit («Qiagen», Германия) согласно инструкции компании-производителя на автоматической станции QIAcube («Qiagen», Германия). Концентрацию суммарной РНК в водном растворе оценивали на спектрофотометре NanoVue Plus («GE Healthcare», Великобритания). Библиотеки были подготовлены с помощью QIAseq miRNA Library Kit согласно стандартным протоколам производителя. Экспрессию микроРНК исследовали с помощью секвенирования на Illumina NextSeq 500 (Illumina NextSeq 500, США).

РЕЗУЛЬТАТЫ

РЕЗУЛЬТАТЫ. Обнаружены 108 дифференциально экспрессирующихся микроРНК  (p <0,05) после поправки на множественность сравнений. МикроРНК разделены на три группы – группа 1 с числом прочтений более 10 в обеих группах сравнения, группа 2 с числом прочтений менее 10 в одной из групп, группа 3 с числом прочтений менее 10 в обеих группах. Для верификации методом количественной ПЦР с обратной транскрипцией (RT-qPCR), планируется использовать следующие микроРНК: miR-383-3p, miR-4290 и miR-6717-5p, экспрессия которых была повышена у пациентов с БИК по сравнению с пациентами из группы АКТГ-ЭС в 46,36 раз (p=0,01), в 6,84 раз (p=0,036) и в 4,49 раз (p=0,031), соответственно,  miR-1203, miR-1229-3p, miR-639, экспрессия которых была снижена у пациентов с БИК в 36,74 раз (p=0,013), в 78,3 раз (p=0,003), в 73,22 раза (p=0,002), соответственно, а также miR-302c-3p,  экспрессия которой была повышена у пациентов с БИК в 92,69 раз (p=0,001).

ЗАКЛЮЧЕНИЕ

ЗАКЛЮЧЕНИЕ. В ходе исследования был значительно расширен список микроРНК-кандидатов для диагностики АКТГ-зависимых форм ЭГ. Необходима валидизация полученных микроРНК в периферической крови на расширенной выборке пациентов методом RT-qPCR.

BACKGROUND

BACKGROUND: For the last decades microRNAs (miR) have proven themselves as novel biomarkers for various types of diseases. Identification of specific circulating microRNA panel that differ patient with Cushing’s disease (CD) and ectopic ACTH syndrome (EAS) could improve the diagnostic procedure.

AIM

AIM: to evaluate the differences in miR levels in plasma samples drained from inferior petrosal sinuses in patients with CD and EAS.

MATERIALS AND METHODS

MATERIALS AND METHODS: single-center, case-control study: we enrolled 24 patients with ACTH-dependent Cushing’s syndrome (CS) requiring bilateral inferior petrosal sinus sampling (BIPSS).  Among them 12 subjects were confirmed as CD (males=2, females=10; median age 46,5 [IR 33,8;53,5]) and 12 as EAS (males=4, females=8, median age 54 [IR 38,75;60,75]). BIPSS was performed through a percutaneous bilateral approach. Once catheters were properly placed, blood samples were withdrawn simultaneously from each petrosal sinus and a peripheral vein. Plasma samples from both sinuses were centrifuged and then stored at -80 C. MiRNA isolation from plasma was carried out by an miRneasy Plasma/Serum Kit (Qiagen, Germany) on the automatic QIAcube station according to the manufacturer protocol. To prevent degradation, we added 1 unit of RiboLock Rnase Inhibitor (Thermo Fisher Scientific, USA) per 1 μL of RNA solution. The concentration of total RNA in the aqueous solution was evaluated on a NanoVue Plus spectrophotometer (GE Healthcare, USA). The libraries were prepared by the QIAseq miRNA Library Kit following the manufacturer standard protocols. MiR expression was then analyzed by sequencing on Illumina NextSeq 500 (Illumina, USA).

RESULTS

RESULTS: 108 miRNAs were differently expressed (p <0,05) in inferior petrosal sinus samples of patients with CD vs EAS. We divided these miRNAs into 3 groups based on the significance of the results. The first group consisted of samples with the highest levels of detected miR in both groups. Four miRNAs were included: miR-1203 was downregulated in CD vs EAS — 36.74 (p=0,013), and three other were upregulated in CD vs EAS: miR-383-3p 46.36 (p=0,01), miR-4290 6.84 (p=0,036), miR-6717-5p 4.49 (p=0,031). This miRs will be validated in larger cohorts using RT-qPCR.

CONCLUSION

CONCLUSION: Plasma miR levels differ in inferior petrosal samples taken from patients with CD vs EAS. These miRs need to be validated by different methods and in peripheral plasma samples in order to be used as potentially non-invasive biomarkers to differentiate ACTH-dependent CS.

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The authors declare that there are no conflicts of interest present.