problendoПроблемы ЭндокринологииProblems of Endocrinology0375-96602308-1430Endocrinology Research Centre10.14341/probl201056119-25problendo-4789Research ArticleСтатьиArticlesКлинические и лабораторные аспекты исследования изоформ пролактина методами ПЭГ-преципитации и ультрафильтрацииClinical and laboratory aspects of investigations into prolactin isoforms by PEG precipitation and ultrafiltration techniquesMel'nichenkoG A-GoncharovN P-DzeranovaL Kdzeranova@endocrincentr.ruDobrachevaA D-BarminaI IBarm-i@mail.ru201015022010561ТОМ 56, №1 (2010)1925Copyright © Mel'nichenko G.A., Goncharov N.P., Dzeranova L.K., Dobracheva A.D., Barmina I.I., 20102010Mel'nichenko G.A., Goncharov N.P., Dzeranova L.K., Dobracheva A.D., Barmina I.I.Mel'nichenko G.A., Goncharov N.P., Dzeranova L.K., Dobracheva A.D., Barmina I.I.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.probl-endojournals.ru/jour/article/view/4789

Изучали изоформы пролактина (ПРЛ) с использованием двух методик - преципитации с полиэтиленгликолем (ПЭГ) и ультрафильтрации (УФ). Проанализированы результаты исследования сывороток у 41 пациентки с гиперпролактинемией. Данные об уровнях мономерного ПРЛ и высокомолекулярных форм ПРЛ, полученные с применением ПЭГ-преципитации и УФ, были различными. Меньшее содержание мономерного ПРЛ после УФ объясняется, по-видимому, отделением в процессе фильтрации не только макропролактина, но и димерной формы гормона. Клинические данные и результаты магнитно-резонанасной томографии (МРТ) головного мозга были сопоставлены с уровнем низкомолекулярного ПРЛ. Значимого различия между содержанием мономерного ПРЛ, выявленного с применением двух различных методик, выявлено не было.

The objective of this work was to study prolactin (PRL) isoforms using two methods, polyethylene glycol (PEG) precipitation and ultrafiltration (UF). Sera from 41 patients with hyperprolactinemia were available for analysis. The two techniques revealed roughly similar levels of monomeric and high-molecular weight PRL variants. The somewhat lower monomeric PRL level following UF may be attributed to separation of not only macroprolactin but also of dimeric forms of the hormone during filtration. The clinical picture of the disease and brain MRI images of the patients were consistent with low-molecular weight PRL levels. Comparison of monomeric PRL levels showed no significant difference between the results of measurements by the two methods.

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The authors declare that there are no conflicts of interest present.