problendoПроблемы ЭндокринологииProblems of Endocrinology0375-96602308-1430Endocrinology Research Centre10.14341/probl201561146-50problendo-7140Research ArticleСтатьиArticlesАссоциация полиморфного маркера G276Т гена адипонектина с абдоминальным ожирением в кыргызской популяцииThe association between adiponectin G276T gene polymorphic marker and abdominal obesity in a Kyrgyz populationИсаковаЖ ТIsakovaZh Tjainagul@mail.ruТалайбековаЭ ТTalaibekovaE T-ЛунеговаО СLunegovaO S-АсамбаеваД АAsambarvaD A-КеримкуловаА СKerimkulovaA S-АлдашевА АAldashevA A-Институт молекулярной биологии и медицины, Бишкек, Кыргызская РеспубликаInstitute of Molecular Biology, Bishkek, Kyrgyz RepublicНациональный центр кардиологии и терапии, Бишкек, Кыргызская РеспубликаNational Centre of Cardiology and Therapy, Bishkek, Kyrgyz Republic201515022015611ТОМ 61, №1 (2015)4650Copyright © Исакова Ж.Т., Талайбекова Э.Т., Лунегова О.С., Асамбаева Д.А., Керимкулова А.С., Алдашев А.А., 20152015Исакова Ж.Т., Талайбекова Э.Т., Лунегова О.С., Асамбаева Д.А., Керимкулова А.С., Алдашев А.А.Isakova Z.T., Talaibekova E.T., Lunegova O.S., Asambarva D.A., Kerimkulova A.S., Aldashev A.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.probl-endojournals.ru/jour/article/view/7140

У пациентов кыргызской национальности изучали взаимосвязь полиморфного локуса G276Т гена адипонектина с развитием абдоминального ожирения (АО). Обследовали 288 человек в возрасте 40-70 лет. 139 пациентов (81 женщина, 58 мужчин) с АО и 149 человек (62 женщин и 87 мужчин) без АО (группа сравнения). Измеряли антропометрические показатели, артериальное давление, уровни глюкозы, инсулина, лептина и оценивали липидный состав крови. Генотипы полиморфизма G276T гена адипонектина (АН) определяли методом ПЦР-ПДРФ. Выявлена взаимосвязь полиморфного маркера G276T гена АН с развитием АО у женщин. У женщин с АО выявлен 31% носителей аллеля Т, в контрольной группе - 17% (χ²=7,89; р=0,005). При генотипах GT+TT и аллеле Т повышается риск развития АО (ОШ=2,5; 95% ДИ: 1,25-4,97 для генотипа и ОШ=2,2; 95% ДИ: 1,26-4,00 для аллеля). У мужчин подобных связей не обнаружено. У женщин с АО и генотипом GT+TT чаще, чем у гомозигот GG, встречались сахарный диабет 2-го типа (64% против 37%; р=0,017), гипертриглицеридемия (41% против 16,2%; р=0,016), высокий уровень глюкозы (7,74±3,3 против 6,52±1,17; р=0,033) и индекс HOMA (3,5±1,7 против 2,63±1,24; р=0,02). Таким образом, полиморфный вариант 276Т гена адипонектина ассоциирован с абдоминальным ожирением, СД2, гипергликемией и гипертриглицеридемией у женщин кыргызской национальности.

A population of ethnic Kyrgyz was examined with a view to elucidating the relationship between the adiponectin G276T gene polymorphic locus and the development of abdominal obesity (AO). The study included 288 subjects at the age between 40 and 70 years. 139 of them (81 women and 58 men) presented with AO while 149 without obesity (62 women and 87 men) constituted the control group. The measured anthropometric parameters included arterial pressure, blood glucose, insulin, and leptin levels, blood lipid composition. Genotypes of adiponectin (AN) G276T gene polymorphism were identified by means of PCR-RFLP analysis. The relationship between the presence of the adiponectin G276T gene polymorphic and the development of abdominal obesity in the women was demonstrated. Specifically, 31% of the women with AO were carriers of T allele compared with 17% in the control group (χ2=7.89; p =0.005). The GT + TT genotype and carriage of T allele were associated with an increased risk of development of abdominal obesity (OR=2.5; 95% CI = 1.25-4.97 for the genotype and OR = 2.2; 95% CI = 1.26-4.000 for the allele). No such relationship was documented among men. The women with AO and GT+TT genotype more frequently than homozygotes presented with type 2 diabetes mellitus (64 and 37% respectively; p=0.017), hypertriglyceridemia (41 and 16.2% respectively; p=0.016), and enhanced blood glucose level (7.74±3.3 and 6.52±1.17; p=0.033). Moreover, their HOMA index was higher than in the homozygotes (3.5±1.7 and 2.63±1.24 respectively; p=0.02). It is concluded that the adiponectin G276T gene polymorphic variant in the women of Kyrgyz ethnicity is associated with abdominal obesity, type 2 diabetes mellitus, hyperglycemia, and hypertriglyceridemia.

абдоминальное ожирениеполиморфизм G276Т гена адипонектинакыргызская популяцияabdominal obesityadiponectin G276T gene polymorphismKyrgyz populationReferencesHaslam DW, James WPT. Obesity. The Lancet. 2005;366(9492):1197-1209. doi: 10.1016/s0140-6736(05)67483-1.Haslam DW, James WPT. Obesity. The Lancet. 2005;366(9492):1197-1209. doi: 10.1016/s0140-6736(05)67483-1.Айтбаев К.А., Хамзамулин Р.О., Мейманалиев Т.М., и др. Эпидемиология ишемической болезни сердца и артериальной гипертонии среди постоянных жителей высокогорья Тянь-Шаня и Памира. Актуальные проблемы кардиологии в зонах нового экономического освоения. - Иркутск; 1978. - С. 27-28.Айтбаев К.А., Хамзамулин Р.О., Мейманалиев Т.М., и др. Эпидемиология ишемической болезни сердца и артериальной гипертонии среди постоянных жителей высокогорья Тянь-Шаня и Памира. Актуальные проблемы кардиологии в зонах нового экономического освоения. - Иркутск; 1978. - С. 27-28.Полупанов А.Н., Халматов А.Н., Алтымышева А.Т., и др. Распространенность сердечно-сосудистых факторов риска среди жителей Кыргызской Республики. Распространенность метаболических факторов риска. // Центрально-Азиатский Медицинский журнал. - 2013. - Т. 19. - №4 - С. 34-38.Полупанов А.Н., Халматов А.Н., Алтымышева А.Т., и др. Распространенность сердечно-сосудистых факторов риска среди жителей Кыргызской Республики. Распространенность метаболических факторов риска. // Центрально-Азиатский Медицинский журнал. - 2013. - Т. 19. - №4 - С. 34-38.Whitehead JP, Richards AA, Hickman IJ, et al. Adiponectin - a key adipokine in the metabolic syndrome. Diabetes, Obesity and Metabolism. 2006;8(3):264-280. doi: 10.1111/j.1463-1326.2005.00510.xWhitehead JP, Richards AA, Hickman IJ, et al. Adiponectin - a key adipokine in the metabolic syndrome. Diabetes, Obesity and Metabolism. 2006;8(3):264-280. doi: 10.1111/j.1463-1326.2005.00510.xYamauchi T, Kamon J, Minokoshi Y, et al. Adiponectin stimulates glucose utilization and fatty-acid oxidation by activating AMP-activated protein kinase. Nat. Med. 2002;8(11):1288-1295. doi: 10.1038/nm788Yamauchi T, Kamon J, Minokoshi Y, et al. Adiponectin stimulates glucose utilization and fatty-acid oxidation by activating AMP-activated protein kinase. Nat. Med. 2002;8(11):1288-1295. doi: 10.1038/nm788Lihn AS, Pedersen SB, Richelsen B. Adiponectin: action, regulation and association to insulin sensitivity. Obes. Rev. 2005;6(1):13-21. doi: 10.1111/j.1467-789X.2005.00159.xLihn AS, Pedersen SB, Richelsen B. Adiponectin: action, regulation and association to insulin sensitivity. Obes. Rev. 2005;6(1):13-21. doi: 10.1111/j.1467-789X.2005.00159.xTakahashi M, Arita Y, Yamagata K, et al. Genomic structure and mutations in adipose-specific gene, adiponectin. Int. J. Obes. 2000;24(7):861-868. doi: 10.1038/sj.ijo.0801244Takahashi M, Arita Y, Yamagata K, et al. Genomic structure and mutations in adipose-specific gene, adiponectin. Int. J. Obes. 2000;24(7):861-868. doi: 10.1038/sj.ijo.0801244Sheng T, Yang K. Adiponectin and its association with insulin resistance and type 2 diabetes. Journal of Genetics and Genomics. 2008;35(6):321-326. doi: 10.1016/s1673-8527(08)60047-8Sheng T, Yang K. Adiponectin and its association with insulin resistance and type 2 diabetes. Journal of Genetics and Genomics. 2008;35(6):321-326. doi: 10.1016/s1673-8527(08)60047-8Siitonen N, Pulkkinen L, Lindström J, et al. Association of ADIPOQ gene variants with body weight, type 2 diabetes and serum adiponectin concentrations: the Finnish Diabetes Prevention Study. BMC Med. Genet. 2011;12(1):5. doi: 10.1186/1471-2350-12-5Siitonen N, Pulkkinen L, Lindström J, et al. Association of ADIPOQ gene variants with body weight, type 2 diabetes and serum adiponectin concentrations: the Finnish Diabetes Prevention Study. BMC Med. Genet. 2011;12(1):5. doi: 10.1186/1471-2350-12-5Cnop M, Havel PJ, Utzschneider KM, et al. Relationship of adiponectin to body fat distribution, insulin sensitivity and plasma lipoproteins: evidence for independent roles of age and sex. Diabetologia. 2003;46(4):459-469. doi: 10.1007/s00125-003-1074-zCnop M, Havel PJ, Utzschneider KM, et al. Relationship of adiponectin to body fat distribution, insulin sensitivity and plasma lipoproteins: evidence for independent roles of age and sex. Diabetologia. 2003;46(4):459-469. doi: 10.1007/s00125-003-1074-zFriedewald WT, Levy RI, Fredrickson DS. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin. Chem. 1972;18(6):499-502.Friedewald WT, Levy RI, Fredrickson DS. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin. Chem. 1972;18(6):499-502.Hotta K, Funahashi T, Bodkin NL, et al. Circulating Concentrations of the Adipocyte Protein Adiponectin Are Decreased in Parallel With Reduced Insulin Sensitivity During the Progression to Type 2 Diabetes in Rhesus Monkeys. Diabetes. 2001;50(5):1126-1133. doi: 10.2337/diabetes.50.5.1126.Hotta K, Funahashi T, Bodkin NL, et al. Circulating Concentrations of the Adipocyte Protein Adiponectin Are Decreased in Parallel With Reduced Insulin Sensitivity During the Progression to Type 2 Diabetes in Rhesus Monkeys. Diabetes. 2001;50(5):1126-1133. doi: 10.2337/diabetes.50.5.1126.Kadowaki T. Adiponectin and adiponectin receptors in insulin resistance, diabetes, and the metabolic syndrome. J. Clin. Invest. 2006;116(7):1784-1792. doi: 10.1172/jci29126.Kadowaki T. Adiponectin and adiponectin receptors in insulin resistance, diabetes, and the metabolic syndrome. J. Clin. Invest. 2006;116(7):1784-1792. doi: 10.1172/jci29126.Lin C-H. Influence of Adiponectin Gene Polymorphisms on Adiponectin Serum Level and Insulin Resistance Index in Taiwanese Metabolic Syndrome Patients. The Chinese Journal of Physiology. 2012;55(6):405-411. doi: 10.4077/cjp.2012.baa081Lin C-H. Influence of Adiponectin Gene Polymorphisms on Adiponectin Serum Level and Insulin Resistance Index in Taiwanese Metabolic Syndrome Patients. The Chinese Journal of Physiology. 2012;55(6):405-411. doi: 10.4077/cjp.2012.baa081Comizio R, Pietrobelli A, Tan YX, et al. Total body lipid and triglyceride response to energy deficit: relevance to body composition models. American Journal of Physiology - Endocrinology and Metabolism. 1998;274(5):E860-E866.Comizio R, Pietrobelli A, Tan YX, et al. Total body lipid and triglyceride response to energy deficit: relevance to body composition models. American Journal of Physiology - Endocrinology and Metabolism. 1998;274(5):E860-E866.Halaas J, Gajiwala K, Maffei M, et al. Weight-reducing effects of the plasma protein encoded by the obese gene. Science. 1995;269(5223):543-546. doi: 10.1126/science.7624777Halaas J, Gajiwala K, Maffei M, et al. Weight-reducing effects of the plasma protein encoded by the obese gene. Science. 1995;269(5223):543-546. doi: 10.1126/science.7624777Yu SY, Ryu HK, Park HJ, et al. Adiponectin gene SNP 276G → T, nutrient intakes, and cardiovascular disease risk in Korean type 2 DM patients. Nutrition research and practice. 2007;1(4):363. doi: 10.4162/nrp.2007.1.4.363Yu SY, Ryu HK, Park HJ, et al. Adiponectin gene SNP 276G → T, nutrient intakes, and cardiovascular disease risk in Korean type 2 DM patients. Nutrition research and practice. 2007;1(4):363. doi: 10.4162/nrp.2007.1.4.363Filippi E, Sentinelli F, Trischitta V, et al. Association of the human adiponectin gene and insulin resistance. Eur. J. Hum. Genet. 2003;12(3):199-205. doi: 10.1038/sj.ejhg.5201120Filippi E, Sentinelli F, Trischitta V, et al. Association of the human adiponectin gene and insulin resistance. Eur. J. Hum. Genet. 2003;12(3):199-205. doi: 10.1038/sj.ejhg.5201120Salmenniemi U, Zacharova J, Ruotsalainen E, et al. Association of Adiponectin Level and Variants in the Adiponectin Gene with Glucose Metabolism, Energy Expenditure, and Cytokines in Offspring of Type 2 Diabetic Patients. The Journal of Clinical Endocrinology & Metabolism. 2005;90(7):4216-4223. doi: 10.1210/jc.2004-2289Salmenniemi U, Zacharova J, Ruotsalainen E, et al. Association of Adiponectin Level and Variants in the Adiponectin Gene with Glucose Metabolism, Energy Expenditure, and Cytokines in Offspring of Type 2 Diabetic Patients. The Journal of Clinical Endocrinology & Metabolism. 2005;90(7):4216-4223. doi: 10.1210/jc.2004-2289Fredriksson J, Carlsson E, Orho-Melander M, et al. A polymorphism in the adiponectin gene influences adiponectin expression levels in visceral fat in obese subjects. Int. J. Obes. 2005;30(2):226-232. doi: 10.1038/sj.ijo.0803138.Fredriksson J, Carlsson E, Orho-Melander M, et al. A polymorphism in the adiponectin gene influences adiponectin expression levels in visceral fat in obese subjects. Int. J. Obes. 2005;30(2):226-232. doi: 10.1038/sj.ijo.0803138.González-Sánchez JL, Zabena CA, Martínez-Larrad MT, et al. An SNP in the Adiponectin Gene Is Associated with Decreased Serum Adiponectin Levels and Risk for Impaired Glucose Tolerance. Obesity. 2005;13(5):807-812. doi: 10.1038/oby.2005.91González-Sánchez JL, Zabena CA, Martínez-Larrad MT, et al. An SNP in the Adiponectin Gene Is Associated with Decreased Serum Adiponectin Levels and Risk for Impaired Glucose Tolerance. Obesity. 2005;13(5):807-812. doi: 10.1038/oby.2005.91Hara K, Boutin P, Mori Y, et al. Genetic Variation in the Gene Encoding Adiponectin Is Associated With an Increased Risk of Type 2 Diabetes in the Japanese Population. Diabetes. 2002;51(2):536-540. doi: 10.2337/diabetes.51.2.536Hara K, Boutin P, Mori Y, et al. Genetic Variation in the Gene Encoding Adiponectin Is Associated With an Increased Risk of Type 2 Diabetes in the Japanese Population. Diabetes. 2002;51(2):536-540. doi: 10.2337/diabetes.51.2.536

The authors declare that there are no conflicts of interest present.