problendoПроблемы ЭндокринологииProblems of Endocrinology0375-96602308-1430Endocrinology Research Centre10.14341/probl201662438-44problendo-7929Research ArticleЭкспериментальная эндокринологияExperimental endocrinologyПолифенольные растительные экстракты: влияние на нарушения углеводного и липидного обмена у лабораторных грызуновPolyphenolic plant extracts: effects on disorders of carbohydrate and lipid metabolism in laboratory animalsМазоВладимир КимовичMazoVladimir K.

доктор биологических наук, профессор, старший научный сотрудник лаборатории алиментарной коррекции нарушений метаболома

PhD

mazo@ion.ruСидороваЮлия СергеевнаSidorovaYuliia S.

кандидат биологических наук, научный сотрудник лаборатории алиментарной коррекции нарушений метаболома

PhD

sidorovaulia28@mail.ruШипелинВладимир АлександровичShipelinVladimir A.

кандидат медицинских наук, научный сотрудник лаборатории алиментарной коррекции нарушений метаболома

PhD

v.shipelin@yandex.ruПетровНикита АлександровичPetrovNikita A.

лаборант-исследователь лаборатории пищевых биотехнологий и специализированных продуктов

PhD

kochetkova@ion.ruКочетковаАлла АлексеевнаKochetkovaAlla A.

доктор технических наук, профессор, заведующая лабораторией пищевых биотехнологий и специализированных продуктов

PhD

kochetkova@ion.ruФГБУН «Федеральный исследовательский центр питания, биотехнологии и безопасности пищи»РоссияFederal Research Centre of Nutrition, Biotechnology and Food SafetyRussian Federation2016100620166243844Copyright © Мазо В.К., Сидорова Ю.С., Шипелин В.А., Петров Н.А., Кочеткова А.А., 20162016Мазо В.К., Сидорова Ю.С., Шипелин В.А., Петров Н.А., Кочеткова А.А.Mazo V.K., Sidorova Y.S., Shipelin V.A., Petrov N.A., Kochetkova A.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.probl-endojournals.ru/jour/article/view/7929

Современной нутрициологией получены убедительные свидетельства участия в метаболизме многих минорных компонентов пищи, не рассматривавшихся ранее в качестве факторов, необходимых для обеспечения жизнедеятельности организма. Одним из инновационных подходов к созданию нового поколения специализированных продуктов для диетотерапии сахарного диабета 2-го типа (СД2) является целенаправленное использование в их составе в качестве «микроингредиентов» растительных минорных биологически активных веществ с доказанным гипогликемическим, гипохолестеринемическим и антиоксидантным действием. Перспективными природными источниками функциональных ингредиентов для диетотерапии и диетопрофилактики СД2 и сопутствующих ему осложнений являются растительные экстракты с высоким содержанием природных полифенольных соединений — флавоноидов. Поскольку с растительной пищей мы потребляем не индивидуальные флавоноиды, а их смеси, с позиций клинической нутрициологии особую значимость приобретают данные об антидиабетических свойствах растительных экстрактов, содержащих различные сочетания флавоноидов. В обзоре кратко обсуждаются основные результаты исследований влияния растительных экстрактов зеленого чая, листьев черники и створок фасоли на нарушения углеводного и липидного обмена у лабораторных грызунов с экспериментальным или генетически обусловленным СД. Отвечающие требованиям доказательной медицины данные подтверждают антиоксидантное, гипогликемическое и/или гиполипидемическое действие полифенольных растительных экстрактов. Сделан вывод о перспективности включения растительных экстрактов флавоноидов в состав специализированных пищевых продуктов, предназначенных для целенаправленной диетологической коррекции и/или профилактики метаболических нарушений при СД2.

Modern nutrition has clear evidence of the involvement into the metabolism of many minor food components, which were not previously discussed as factors necessary for life support of health and ill human. One of the innovative approaches to the creation of a new generation of specialized food products for the dietary treatment of type 2 diabetes is a targeted use in their composition plant minor biologically active food substances with proven significant hypoglycemic, cholesterol-lowering and antioxidant action as a «micro-ingredients». Promising sources of natural functional food ingredients for the dietary treatment and prevention of type 2 diabetes and its attendant complications are plant extracts with a high content of natural polyphenolic compounds. The natural polyphenolic compounds include flavonoids, obligate food antioxidants what is the reason of their hypoglycemic and/or lipid-lowering effects. However, we consume no individual flavonoids, but their mixtures, with plant food. Data about antidiabetic properties of plant extracts containing various combinations of flavonoids are very important in the framework of the problem of dietary correction and prevention of type 2 diabetes from the position of clinical nutrition. The review briefly discusses the effect of green tea, bilberry leaves and bean coats plant extracts on disorders of carbohydrate and lipid metabolism in laboratory animals with experimentally or genetically determined diabetes. The presented analysis of publications shows that the results of experimental studies in vitro and in vivo confirm the antioxidant, hypoglycemic and/or hypolipidemic effect of polyphenolic plant extracts. It was concluded that the inclusion of plant extracts of flavonoids in the specialized food products for targeted nutritional correction and/or prevention of metabolic disorders of type 2 diabetes is a promising direction.

диабет 2 типаклиническая нутрициологияполифенолыфлавоноидырастительные экстрактыобзорtype 2 diabetesclinical nutritiologypolyphenolsflavonoidsplant extractsreviewГрант Российского научного фонда (проект № 14-36-00041)The grant of the Russian Science Foundation (Project № 14-36-00041)ReferencesBohn T. Dietary factors affecting polyphenol bioavailability. Nutr Rev. 2014;72(7):429-452. doi: 10.1111/nure.12114Bohn T. Dietary factors affecting polyphenol bioavailability. Nutr Rev. 2014;72(7):429-452. doi: 10.1111/nure.12114Tarahovsky YS, Muzafarov EN, Kim YA. Rafts making and rafts braking: how plant flavonoids may control membrane heterogeneity. Mol Cell Biochem. 2008;314(1-2):65-71. doi: 10.1007/s11010-008-9766-9Tarahovsky YS, Muzafarov EN, Kim YA. Rafts making and rafts braking: how plant flavonoids may control membrane heterogeneity. Mol Cell Biochem. 2008;314(1-2):65-71. doi: 10.1007/s11010-008-9766-9Han MK. Epigallocatechin gallate, a constituent of green tea, suppresses cytokine-induced pancreatic beta-cell damage. Exp Mol Med. 2003;35(2):136-139. doi: 10.1038/emm.2003.19Han MK. Epigallocatechin gallate, a constituent of green tea, suppresses cytokine-induced pancreatic beta-cell damage. Exp Mol Med. 2003;35(2):136-139. doi: 10.1038/emm.2003.19Zhang Z, Ding Y, Dai X, et al. Epigallocatechin-3-gallate protects pro-inflammatory cytokine induced injuries in insulin-producing cells through the mitochondrial pathway. Eur J Pharmacol. 2011;670(1):311-316. doi: 10.1016/j.ejphar.2011.08.033Zhang Z, Ding Y, Dai X, et al. Epigallocatechin-3-gallate protects pro-inflammatory cytokine induced injuries in insulin-producing cells through the mitochondrial pathway. Eur J Pharmacol. 2011;670(1):311-316. doi: 10.1016/j.ejphar.2011.08.033Hsu CH, Liao YL, Lin SC, et sl. Does supplementation with green tea extract improve insulin resistance in obese type 2 diabetics? A randomized, double-blind, and placebo-controlled clinical trial. Altern Med Rev. 2011;16(2):157-163.Hsu CH, Liao YL, Lin SC, et sl. Does supplementation with green tea extract improve insulin resistance in obese type 2 diabetics? A randomized, double-blind, and placebo-controlled clinical trial. Altern Med Rev. 2011;16(2):157-163.Rains TM, Agarwal S, Maki KC. Antiobesity effects of green tea catechins: a mechanistic review. J Nutr Biochem. 2011;22(1):1-7. doi: 10.1016/j.jnutbio.2010.06.006Rains TM, Agarwal S, Maki KC. Antiobesity effects of green tea catechins: a mechanistic review. J Nutr Biochem. 2011;22(1):1-7. doi: 10.1016/j.jnutbio.2010.06.006Zheng XX, Xu YL, Li SH, et al. Effects of green tea catechins with or without caffeine on glycemic control in adults: a metaanalysis of randomized controlled trials. Am J Clin Nutr. 2013;97(4):750-762. doi: 10.3945/ajcn.111.032573Zheng XX, Xu YL, Li SH, et al. Effects of green tea catechins with or without caffeine on glycemic control in adults: a metaanalysis of randomized controlled trials. Am J Clin Nutr. 2013;97(4):750-762. doi: 10.3945/ajcn.111.032573Liu CY, Huang CJ, Huang LH, et al. Effects of green tea extract on insulin resistance and lucagon-like peptide 1 in patients with type 2 diabetes and lipid abnormalities: a randomized, double-blinded, and placebo-controlled trial. PLoS One. 2014;9(3):E91163. doi: 10.1371/journal.pone.0091163Liu CY, Huang CJ, Huang LH, et al. Effects of green tea extract on insulin resistance and lucagon-like peptide 1 in patients with type 2 diabetes and lipid abnormalities: a randomized, double-blinded, and placebo-controlled trial. PLoS One. 2014;9(3):E91163. doi: 10.1371/journal.pone.0091163Wolfram S, Raederstorff D, Preller M, et al. Epigallocatechin gallate supplementation alleviates diabetes in rodents. J Nutr. 2006;136(10):2512-2518.Wolfram S, Raederstorff D, Preller M, et al. Epigallocatechin gallate supplementation alleviates diabetes in rodents. J Nutr. 2006;136(10):2512-2518.Song EK, Hur H, Han MK. Epigallocatechin gallate prevents autoimmune diabetes induced by multiple low doses of streptozotocin in mice. Arch Pharm Res. 2003;26(7):559-563. doi: 10.1007/Bf02976881Song EK, Hur H, Han MK. Epigallocatechin gallate prevents autoimmune diabetes induced by multiple low doses of streptozotocin in mice. Arch Pharm Res. 2003;26(7):559-563. doi: 10.1007/Bf02976881Tsuneki H, Ishizuka M, Terasawa M, et al. Effect of green tea on blood glucose levels and serum proteomic patterns in diabetic (db/db) mice and on glucose metabolism in healthy humans BMC. Pharmacol. 2004;4(1):18. doi: 10.1186/1471-2210-4-18Tsuneki H, Ishizuka M, Terasawa M, et al. Effect of green tea on blood glucose levels and serum proteomic patterns in diabetic (db/db) mice and on glucose metabolism in healthy humans BMC. Pharmacol. 2004;4(1):18. doi: 10.1186/1471-2210-4-18Wu L-Y, Juan C-C, Hwang LS, et al. Green tea supplementation ameliorates insulin resistance and increases glucose transporter IV content in a fructose-fed rat model. Eur J Nutr. 2004;43(2):116-124. doi: 10.1007/S00394-004-0450-xWu L-Y, Juan C-C, Hwang LS, et al. Green tea supplementation ameliorates insulin resistance and increases glucose transporter IV content in a fructose-fed rat model. Eur J Nutr. 2004;43(2):116-124. doi: 10.1007/S00394-004-0450-xBaluchnejadmojarad T, Roghani M. Chronic oral epigallocatechin-gallate alleviates streptozotocin-induced diabetic neuropathic hyperalgesia in rat: involvement of oxidative stress. Iran J Pharm Res. 2012;11(4):1243-1253. PMC3813147Baluchnejadmojarad T, Roghani M. Chronic oral epigallocatechin-gallate alleviates streptozotocin-induced diabetic neuropathic hyperalgesia in rat: involvement of oxidative stress. Iran J Pharm Res. 2012;11(4):1243-1253. PMC3813147Roghani M, Baluchnejadmojarad T. Hypoglycemic and hypolipidemic effect and antioxidant activity of chronic epigallocatechin-gallate in streptozotocin-diabetic rats. Pathophysiology. 2010;17(1):55-59. doi: 10.1016/j.pathophys.2009.07.004Roghani M, Baluchnejadmojarad T. Hypoglycemic and hypolipidemic effect and antioxidant activity of chronic epigallocatechin-gallate in streptozotocin-diabetic rats. Pathophysiology. 2010;17(1):55-59. doi: 10.1016/j.pathophys.2009.07.004Potenza MA, Marasciulo FL, Tarquinio M, et al. EGCG, a green tea polyphenol, improves endothelial function and insulin sensitivity, reduces blood pressure, and protects against myocardial I/R injury in SHR. Am J Physiol Endocrinol Metab. 2007;292(5):E1378-E1387. doi: 10.1152/ajpendo.00698.2006Potenza MA, Marasciulo FL, Tarquinio M, et al. EGCG, a green tea polyphenol, improves endothelial function and insulin sensitivity, reduces blood pressure, and protects against myocardial I/R injury in SHR. Am J Physiol Endocrinol Metab. 2007;292(5):E1378-E1387. doi: 10.1152/ajpendo.00698.2006Yoon SP, Maeng YH, Hong R, et al. Protective effects of epigallocatechin gallate (EGCG) on streptozotocin-induced diabetic nephropathy in mice. Acta Histochem. 2014;116(8):1210-1215. doi: 10.1016/j.acthis.2014.07.003Yoon SP, Maeng YH, Hong R, et al. Protective effects of epigallocatechin gallate (EGCG) on streptozotocin-induced diabetic nephropathy in mice. Acta Histochem. 2014;116(8):1210-1215. doi: 10.1016/j.acthis.2014.07.003Isbrucker RA, Edwards JA, Wolz E, et al. Safety studies on epigallocatechin gallate (EGCG) preparations. Part 2: dermal, acute and short-term toxicity studies. Food Chem Toxicol. 2006;44(5):636-650. doi: 10.1016/j.fct.2005.11.003Isbrucker RA, Edwards JA, Wolz E, et al. Safety studies on epigallocatechin gallate (EGCG) preparations. Part 2: dermal, acute and short-term toxicity studies. Food Chem Toxicol. 2006;44(5):636-650. doi: 10.1016/j.fct.2005.11.003Isbrucker RA, Edwards JA, Wolz E, et al. Safety studies on epigallocatechin gallate (EGCG) preparations. Part 3: teratogenicity and reproductive toxicity studies in rats. Food Chem Toxicol. 2006;44(5):651-661. doi: 10.1016/j.fct.2005.11.002Isbrucker RA, Edwards JA, Wolz E, et al. Safety studies on epigallocatechin gallate (EGCG) preparations. Part 3: teratogenicity and reproductive toxicity studies in rats. Food Chem Toxicol. 2006;44(5):651-661. doi: 10.1016/j.fct.2005.11.002Martineau LC, Couture A, Spoor D, et al. Anti-diabetic properties of the Canadian lowbush blueberry vaccinium angustifolium Ait. Phytomedicine. 2006;13(9-10):612-623. doi: 10.1016/j.phymed.2006.08.005Martineau LC, Couture A, Spoor D, et al. Anti-diabetic properties of the Canadian lowbush blueberry vaccinium angustifolium Ait. Phytomedicine. 2006;13(9-10):612-623. doi: 10.1016/j.phymed.2006.08.005Grace MH, Ribnicky DM, Kuhn P, et al. Hypoglycemic activity of a novel anthocyanin-rich formulation from lowbush blueberry, vaccinium angustifolium aiton. Phytomedicine. 2009;16(5):406-415. doi: 10.1016/j.phymed.2009.02.018Grace MH, Ribnicky DM, Kuhn P, et al. Hypoglycemic activity of a novel anthocyanin-rich formulation from lowbush blueberry, vaccinium angustifolium aiton. Phytomedicine. 2009;16(5):406-415. doi: 10.1016/j.phymed.2009.02.018Ehlenfeldt MK, Prior RL. Oxygen radical absorbance capacity (ORAC) and phenolic and anthocyanin concentrations in fruit and leaf tissues of highbush blueberry. J Agric Food Chem. 2001;49(5):2222-2227. doi: 10.1021/jf0013656Ehlenfeldt MK, Prior RL. Oxygen radical absorbance capacity (ORAC) and phenolic and anthocyanin concentrations in fruit and leaf tissues of highbush blueberry. J Agric Food Chem. 2001;49(5):2222-2227. doi: 10.1021/jf0013656Cravotto G, Boffa L, Genzini L, Garella D. Phytotherapeutics: an evaluation of the potential of 1000 plants. J Clin Pharm Ther. 2010;35(1):11-48. doi: 10.1111/j.1365-2710.2009.01096.xCravotto G, Boffa L, Genzini L, Garella D. Phytotherapeutics: an evaluation of the potential of 1000 plants. J Clin Pharm Ther. 2010;35(1):11-48. doi: 10.1111/j.1365-2710.2009.01096.xUpton R, Editor. Bilberry fruit vaccinium myrtillus L. Standards Of Analysis, Quality Control, And Therapeutics. Santa Cruz, Ca: American Herbal Pharmacopoeia And Therapeutic Compendium. 2001.Upton R, Editor. Bilberry fruit vaccinium myrtillus L. Standards Of Analysis, Quality Control, And Therapeutics. Santa Cruz, Ca: American Herbal Pharmacopoeia And Therapeutic Compendium. 2001.Burdulis D, Sarkinas A, Jasutiene I, et al. Comparative study of anthocyanin composition, antimicrobial and antioxidant activity in bilberry (Vaccinium myrtillus L.) and blueberry (Vaccinium corymbosum L.) Fruits. Acta Pol Pharm. 2009;66(4):399-408.Burdulis D, Sarkinas A, Jasutiene I, et al. Comparative study of anthocyanin composition, antimicrobial and antioxidant activity in bilberry (Vaccinium myrtillus L.) and blueberry (Vaccinium corymbosum L.) Fruits. Acta Pol Pharm. 2009;66(4):399-408.Seeram NP. Berry Fruits: compositional elements, biochemical activities, and the impact of their intake on human health, performance, and disease. J Agric Food Chem. 2008;56(3):627-629. doi: 10.1021/jf071988kSeeram NP. Berry Fruits: compositional elements, biochemical activities, and the impact of their intake on human health, performance, and disease. J Agric Food Chem. 2008;56(3):627-629. doi: 10.1021/jf071988kWu X, Prior RL. Systematic identification and characterization of anthocyanins by HPLC-ESI-MS/MS in common foods in the United States: fruits and berries. J Agric Food Chem. 2005;53(7):2589-2599. doi: 10.1021/jf048068bWu X, Prior RL. Systematic identification and characterization of anthocyanins by HPLC-ESI-MS/MS in common foods in the United States: fruits and berries. J Agric Food Chem. 2005;53(7):2589-2599. doi: 10.1021/jf048068bSakakibara H, Ogawa T, Koyanagi A, et al. Distribution and excretion of bilberry anthocyanins [corrected] in mice. J Agric Food Chem. 2009;57(17):7681-7686. doi: 10.1021/jf901341bSakakibara H, Ogawa T, Koyanagi A, et al. Distribution and excretion of bilberry anthocyanins [corrected] in mice. J Agric Food Chem. 2009;57(17):7681-7686. doi: 10.1021/jf901341bWatson EM. Some observations on the effect of blueberry leaf extract in diabetes mellitus. Can Med Assoc J. 1928;19(2):166-171.Watson EM. Some observations on the effect of blueberry leaf extract in diabetes mellitus. Can Med Assoc J. 1928;19(2):166-171.Valentová K, Ulrichová J, Cvak L, Šimánek V. Cytoprotective effect of a bilberry extract against oxidative damage of rat hepatocytes. Food Chem. 2007;101(3):912-917. doi: 10.1016/j.foodchem.2006.02.038Valentová K, Ulrichová J, Cvak L, Šimánek V. Cytoprotective effect of a bilberry extract against oxidative damage of rat hepatocytes. Food Chem. 2007;101(3):912-917. doi: 10.1016/j.foodchem.2006.02.038Lala G, Malik M, Zhao C, et al. Anthocyanin-rich extracts inhibit multiple biomarkers of colon cancer in rats. Nutr Cancer. 2006;54(1):84-93. doi: 10.1207/s15327914nc5401_10Lala G, Malik M, Zhao C, et al. Anthocyanin-rich extracts inhibit multiple biomarkers of colon cancer in rats. Nutr Cancer. 2006;54(1):84-93. doi: 10.1207/s15327914nc5401_10Kolosova NG, Shcheglova TV, Sergeeva SV, Loskutova LV. Long-term antioxidant supplementation attenuates oxidative stress markers and cognitive deficits in senescent-accelerated oxys rats. Neurobiol Aging. 2006;27(9):1289-1297. doi: 10.1016/j.neurobiolaging.2005.07.022Kolosova NG, Shcheglova TV, Sergeeva SV, Loskutova LV. Long-term antioxidant supplementation attenuates oxidative stress markers and cognitive deficits in senescent-accelerated oxys rats. Neurobiol Aging. 2006;27(9):1289-1297. doi: 10.1016/j.neurobiolaging.2005.07.022Song Y, Park HJ, Kang SN, et al. Blueberry peel extracts inhibit adipogenesis in 3T3-L1 cells and reduce high-fat diet-induced obesity. PLos One. 2013;8(7):E69925. doi: 10.1371/journal.pone.0069925Song Y, Park HJ, Kang SN, et al. Blueberry peel extracts inhibit adipogenesis in 3T3-L1 cells and reduce high-fat diet-induced obesity. PLos One. 2013;8(7):E69925. doi: 10.1371/journal.pone.0069925Brader L, Overgaard A, Christensen LP, et al. Polyphenol-rich bilberry ameliorates total cholesterol and LDL-cholesterol when implemented in the diet of zucker diabetic fatty rats. Rev Diabet Stud. 2013;10(4):270-282. doi: 10.1900/rds.2013.10.270Brader L, Overgaard A, Christensen LP, et al. Polyphenol-rich bilberry ameliorates total cholesterol and LDL-cholesterol when implemented in the diet of zucker diabetic fatty rats. Rev Diabet Stud. 2013;10(4):270-282. doi: 10.1900/rds.2013.10.270Takikawa M, Inoue S, Horio F, Tsuda T. Dietary anthocyanin-rich bilberry extract ameliorates hyperglycemia and insulin sensitivity via activation of AMP-cctivated protein kinase in diabetic mice. J Nutr. 2010;140(3):527-533. doi: 10.3945/jn.109.118216Takikawa M, Inoue S, Horio F, Tsuda T. Dietary anthocyanin-rich bilberry extract ameliorates hyperglycemia and insulin sensitivity via activation of AMP-cctivated protein kinase in diabetic mice. J Nutr. 2010;140(3):527-533. doi: 10.3945/jn.109.118216Hardie DG. Role of AMP-ativated protein kinase in the metabolic syndrome and in heart disease. Febs Lett. 2008;582(1):81-89. doi: 10.1016/j.febslet.2007.11.018Hardie DG. Role of AMP-ativated protein kinase in the metabolic syndrome and in heart disease. Febs Lett. 2008;582(1):81-89. doi: 10.1016/j.febslet.2007.11.018Mykkanen OT, Kalesnykas G, Adriaens M, et al. Bilberries potentially alleviate stress-related retinal gene expression induced by a high-fat diet in mice. Mol Vis. 2012;18:2338-2351. PMC3444297Mykkanen OT, Kalesnykas G, Adriaens M, et al. Bilberries potentially alleviate stress-related retinal gene expression induced by a high-fat diet in mice. Mol Vis. 2012;18:2338-2351. PMC3444297Roopchand DE, Grace MH, Kuhn P, et al. Efficient sorption of polyphenols to soybean flour enables natural fortification of foods. Food Chem. 2012;131(4):1193-1200. doi: 10.1016/j.foodchem.2011.09.103Roopchand DE, Grace MH, Kuhn P, et al. Efficient sorption of polyphenols to soybean flour enables natural fortification of foods. Food Chem. 2012;131(4):1193-1200. doi: 10.1016/j.foodchem.2011.09.103Roopchand DE, Kuhn P, Poulev A, et al. Biochemical analysis and in vivo hypoglycemic activity of a grape polyphenol-soybean flour complex. J Agric Food Chem. 2012;60(36):8860-8865. doi: 10.1021/jf300232hRoopchand DE, Kuhn P, Poulev A, et al. Biochemical analysis and in vivo hypoglycemic activity of a grape polyphenol-soybean flour complex. J Agric Food Chem. 2012;60(36):8860-8865. doi: 10.1021/jf300232hRoopchand DE, Kuhn P, Rojo LE, et al. Blueberry polyphenol-enriched soybean flour reduces hyperglycemia, body weight gain and serum cholesterol in mice. Pharmacol Res. 2013;68(1):59-67. doi: 10.1016/j.phrs.2012.11.008Roopchand DE, Kuhn P, Rojo LE, et al. Blueberry polyphenol-enriched soybean flour reduces hyperglycemia, body weight gain and serum cholesterol in mice. Pharmacol Res. 2013;68(1):59-67. doi: 10.1016/j.phrs.2012.11.008Jeong HR, Jo YN, Jeong JH, et al. Blueberry (Vaccinium virgatum) leaf extracts protect against abeta-induced cytotoxicity and cognitive impairment. J Med Food. 2013;16(11):968-976. doi: 10.1089/jmf.2013.2881Jeong HR, Jo YN, Jeong JH, et al. Blueberry (Vaccinium virgatum) leaf extracts protect against abeta-induced cytotoxicity and cognitive impairment. J Med Food. 2013;16(11):968-976. doi: 10.1089/jmf.2013.2881Lee IC, Kim DY, Choi BY. Antioxidative activity of blueberry leaf extract prevents high-fat diet-induced obesity in C57BL/6 mice. J Cancer Prev. 2014;19(3):209-215. doi: 10.15430/jcp.2014.19.3.209Lee IC, Kim DY, Choi BY. Antioxidative activity of blueberry leaf extract prevents high-fat diet-induced obesity in C57BL/6 mice. J Cancer Prev. 2014;19(3):209-215. doi: 10.15430/jcp.2014.19.3.209Nagao K, Higa K, Shirouchi B, et al. Effect of Vaccinium ashei reade leaves on lipid metabolism in Otsuka long-evans Tokushima fatty rats. Biosci Biotechnol Biochem. 2008;72(6):1619-1622. doi: 10.1271/bbb.80036Nagao K, Higa K, Shirouchi B, et al. Effect of Vaccinium ashei reade leaves on lipid metabolism in Otsuka long-evans Tokushima fatty rats. Biosci Biotechnol Biochem. 2008;72(6):1619-1622. doi: 10.1271/bbb.80036Inoue N, Nagao K, Nomura S, et al. Effect of Vaccinium ashei reade leaf extracts on lipid metabolism in obese OLETF rats. Biosci Biotechnol Biochem. 2011;75(12):2304-2308. doi: 10.1271/bbb.110451Inoue N, Nagao K, Nomura S, et al. Effect of Vaccinium ashei reade leaf extracts on lipid metabolism in obese OLETF rats. Biosci Biotechnol Biochem. 2011;75(12):2304-2308. doi: 10.1271/bbb.110451Yuji K, Sakaida H, Kai T, et al. Effect of dietary blueberry (vaccinium ashei reade) leaves on serum and hepatic lipid levels in rats. Journal of Oleo Science. 2013;62(2):89-96. doi: 10.5650/jos.62.89Yuji K, Sakaida H, Kai T, et al. Effect of dietary blueberry (vaccinium ashei reade) leaves on serum and hepatic lipid levels in rats. Journal of Oleo Science. 2013;62(2):89-96. doi: 10.5650/jos.62.89Beninger CW, Hosfield GL. Antioxidant activity of extracts, condensed tannin fractions, and pure flavonoids fromphaseolus vulgaris. Seed coat color genotypes. J Agric Food Chem. 2003;51(27):7879-7883. doi: 10.1021/jf0304324Beninger CW, Hosfield GL. Antioxidant activity of extracts, condensed tannin fractions, and pure flavonoids fromphaseolus vulgaris. Seed coat color genotypes. J Agric Food Chem. 2003;51(27):7879-7883. doi: 10.1021/jf0304324Aparicio-Fernández X, Manzo-Bonilla L, Loarca-Piña GF. Comparison of antimutagenic activity of phenolic compounds in newly harvested and stored common beans phaseolus vulgaris against aflatoxin B1. J Food Sci. 2005;70(1):S73-S78. doi: 10.1111/j.1365-2621.2005.tb09068.xAparicio-Fernández X, Manzo-Bonilla L, Loarca-Piña GF. Comparison of antimutagenic activity of phenolic compounds in newly harvested and stored common beans phaseolus vulgaris against aflatoxin B1. J Food Sci. 2005;70(1):S73-S78. doi: 10.1111/j.1365-2621.2005.tb09068.xZhao J, Fan Z, Zhou W. Research progress on health functions of red adzuki bean. Journal of Agricultural Science and Technology. 2009;11(3):46-50.Zhao J, Fan Z, Zhou W. Research progress on health functions of red adzuki bean. Journal of Agricultural Science and Technology. 2009;11(3):46-50.Xavier-Filho J, Oliveira AEA, Silva LBD, et al. Plant insulin or glucokinin: a conflicting issue. Brazilian Journal of Plant Physiology. 2003;15(2). doi: 10.1590/s1677-04202003000200002Xavier-Filho J, Oliveira AEA, Silva LBD, et al. Plant insulin or glucokinin: a conflicting issue. Brazilian Journal of Plant Physiology. 2003;15(2). doi: 10.1590/s1677-04202003000200002Ranilla LG, Kwon Y-I, Genovese MI, et al. Effect of thermal treatment on phenolic compounds and functionality linked to type 2 diabetes and hypertension management of peruvian and Brazilian bean cultivars (Phaseolus vulgaris L.). Usingin Vitromethods. J Food Biochem. 2010;34(2):329-355. doi: 10.1111/j.1745-4514.2009.00281.xRanilla LG, Kwon Y-I, Genovese MI, et al. Effect of thermal treatment on phenolic compounds and functionality linked to type 2 diabetes and hypertension management of peruvian and Brazilian bean cultivars (Phaseolus vulgaris L.). Usingin Vitromethods. J Food Biochem. 2010;34(2):329-355. doi: 10.1111/j.1745-4514.2009.00281.xRanilla LG, Genovese MI, Lajolo FM. Polyphenols and antioxidant capacity of seed coat and cotyledon from Brazilian and peruvian bean cultivars (Phaseolus vulgaris L.). J Agric Food Chem. 2007;55(1):90-98. doi: 10.1021/jf062785jRanilla LG, Genovese MI, Lajolo FM. Polyphenols and antioxidant capacity of seed coat and cotyledon from Brazilian and peruvian bean cultivars (Phaseolus vulgaris L.). J Agric Food Chem. 2007;55(1):90-98. doi: 10.1021/jf062785jSaks V, Cao D, Li H, et al. Antioxidant properties of the mung bean flavonoids on alleviating heat stress. PLos One. 2011;6(6):E21071. doi: 10.1371/journal.pone.0021071Saks V, Cao D, Li H, et al. Antioxidant properties of the mung bean flavonoids on alleviating heat stress. PLos One. 2011;6(6):E21071. doi: 10.1371/journal.pone.0021071Beninger CW, Hosfield GL, Nair MG. Flavonol glycosides from the seed coat of a new manteca-type dry bean (Phaseolus vulgaris L.). J Agric Food Chem. 1998;46(8):2906-2910. doi: 10.1021/jf9801522Beninger CW, Hosfield GL, Nair MG. Flavonol glycosides from the seed coat of a new manteca-type dry bean (Phaseolus vulgaris L.). J Agric Food Chem. 1998;46(8):2906-2910. doi: 10.1021/jf9801522Aparicio-Fernandez X, Yousef GG, Loarca-Pina G, et al. Characterization of polyphenolics in the seed coat of black jamapa bean (Phaseolus Vulgaris L.). J Agric Food Chem. 2005;53(11):4615-4622. doi: 10.1021/jf047802oAparicio-Fernandez X, Yousef GG, Loarca-Pina G, et al. Characterization of polyphenolics in the seed coat of black jamapa bean (Phaseolus Vulgaris L.). J Agric Food Chem. 2005;53(11):4615-4622. doi: 10.1021/jf047802oJang Y-H, Kang M-J, Choe E-O, et al. Mung bean voat ameliorates hyperglycemia and the antioxidant status in type 2 diabetic db/db mice. Food Sci Biotech. 2013;23(1):247-252. doi: 10.1007/s10068-014-0034-3Jang Y-H, Kang M-J, Choe E-O, et al. Mung bean voat ameliorates hyperglycemia and the antioxidant status in type 2 diabetic db/db mice. Food Sci Biotech. 2013;23(1):247-252. doi: 10.1007/s10068-014-0034-3Yao Y, Chen F, Wang M, et al. Antidiabetic activity of mung bean extracts in diabetic KK-aymice. J Agric Food Chem. 2008; 56(19):8869-8873. doi: 10.1021/jf8009238Yao Y, Chen F, Wang M, et al. Antidiabetic activity of mung bean extracts in diabetic KK-aymice. J Agric Food Chem. 2008; 56(19):8869-8873. doi: 10.1021/jf8009238Wang JY, Zhu C, Qian TW, et al. Extracts of black bean peel and pomegranate peel ameliorate oxidative stress induced hyperglycemia in mice. Exp Ther Med. 2014. doi: 10.3892/etm.2014.2040Wang JY, Zhu C, Qian TW, et al. Extracts of black bean peel and pomegranate peel ameliorate oxidative stress induced hyperglycemia in mice. Exp Ther Med. 2014. doi: 10.3892/etm.2014.2040Saks V, Cao D, Li H, et al. Antioxidant properties of the mung bean flavonoids on alleviating heat stress. PLos One. 2011;6(6):E21071. doi: 10.1371/journal.pone.0021071Saks V, Cao D, Li H, et al. Antioxidant properties of the mung bean flavonoids on alleviating heat stress. PLos One. 2011;6(6):E21071. doi: 10.1371/journal.pone.0021071Мазо В.К., Мурашев А.Н., Сидорова Ю.С. и др. Генетические модели диабета 2-го типа на крысах для оценки эффективности минорных биологически активных веществ пищи // Вопросы Питания. — 2014. — Т.83. — №6. — С.25-31. [Mazo VK, Murashev AN, Sidorova YS, et al. Genetic rat models of type 2 diabetes for evaluation the effectiveness of minor biologically active food substances.Problems of Nutrition. 2014;83(6):25-31. (In Russ.)].Мазо В.К., Мурашев А.Н., Сидорова Ю.С. и др. Генетические модели диабета 2-го типа на крысах для оценки эффективности минорных биологически активных веществ пищи // Вопросы Питания. — 2014. — Т.83. — №6. — С.25-31. [Mazo VK, Murashev AN, Sidorova YS, et al. Genetic rat models of type 2 diabetes for evaluation the effectiveness of minor biologically active food substances.Problems of Nutrition. 2014;83(6):25-31. (In Russ.)].Мазо В.К., Сидорова Ю.С., Кочеткова А.А. Генетические модели сахарного диабета 2-го типа на мышах для оценки эффективности минорных биологически активных веществ пищи. // Вопросы Питания. — 2015. — Т.84. — №6. — C.63-68. [Mazo VK, Sidorova YuS, Kochetkova AA. Genetic mice models of type 2 diabetes for evaluation of the effectiveness of minor biologically active food substances. Problems of Nutrition. 2015;84(6):63-68. (In Russ.) doi: 10.14341/probl201662438-44Мазо В.К., Сидорова Ю.С., Кочеткова А.А. Генетические модели сахарного диабета 2-го типа на мышах для оценки эффективности минорных биологически активных веществ пищи. // Вопросы Питания. — 2015. — Т.84. — №6. — C.63-68. [Mazo VK, Sidorova YuS, Kochetkova AA. Genetic mice models of type 2 diabetes for evaluation of the effectiveness of minor biologically active food substances. Problems of Nutrition. 2015;84(6):63-68. (In Russ.) doi: 10.14341/probl201662438-44

The authors declare that there are no conflicts of interest present.