DEVELOPMENT OF ENZYMOIMMUNOASSAY FOR THE DETERMINATION OF TESTOSTERONE AND ITS USE FOR THE EVALUATION OF GONADAL ANDROGENIC ACTIVITY

N P Goncharov; N D Goncharova; S G Anua

doi:10.14341/probl199036644-48

DEVELOPMENT OF ENZYMOIMMUNOASSAY FOR THE DETERMINATION OF TESTOSTERONE AND ITS USE FOR THE EVALUATION OF GONADAL ANDROGENIC ACTIVITY

N P Goncharov, N D Goncharova, S G Anua

https://doi.org/10.14341/probl199036644-48

Abstract

The authors have summed up the results of development of enzymoimmunoassay (EIA) for the determination of testosterone (T) in human and animal blood plasma. The method is based upon the use of rabbit antiserum to testosterone-3-(0-carboxymethyl) oxim with bovine serum albumin as a binding system, a conjugate of T with horse-radish peroxidase as a marker, and the formation of the antigen-antibody complex was registered with a spectrophotometer of Cf-4a, Cf-16 and Cf-26 types. The method is highly sensitive (6.25 pg/test), specific and is characterized by high indices of precision. Close correlation between EIA and RIA results for the determination of a T level in human and simian plasma under physiological conditions as well as in some pathological conditions, associated with disturbed production of androgens, indicates the correctness of this method.

About the Authors

N P Goncharov

N D Goncharova

НИИ экспериментальной патологии и терапии АМН СССР

S G Anua

References

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3. МЗ СССР ТУ 42-17-86 Набор реактивов для радиоиммунологического определения тестостерона в плазме крови/Гончаров Н. П., Кация Г. В., Гончарова Н. Д. и др.

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Review

For citations:

Goncharov N.P., Goncharova N.D., Anua S.G. DEVELOPMENT OF ENZYMOIMMUNOASSAY FOR THE DETERMINATION OF TESTOSTERONE AND ITS USE FOR THE EVALUATION OF GONADAL ANDROGENIC ACTIVITY. Problems of Endocrinology. 1990;36(6):44-48. https://doi.org/10.14341/probl199036644-48

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND 4.0).

ISSN 0375-9660 (Print)
ISSN 2308-1430 (Online)

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Problems of Endocrinology

DEVELOPMENT OF ENZYMOIMMUNOASSAY FOR THE DETERMINATION OF TESTOSTERONE AND ITS USE FOR THE EVALUATION OF GONADAL ANDROGENIC ACTIVITY

Abstract

About the Authors

References

Review

For citations:

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