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DIRECT RADIOIMMUNOASSAY OF BLOOD PLASMA DEHYDROEPIANDROSTERONE SULFATE AND ASSESSMENT OF THE ADRENOCORTICAL ANDROGENIC FUNCTION

https://doi.org/10.14341/probl199339326-29

Abstract

The author suggests a direct radioimmunoassay of dehydroepiandrosterone sulfate (DEA-S) in 0.05-2 μ1 of human blood plasma, making use of an antiserum prepared by rabbit immunization with DEA-S hemisuccinate conjugate with bovine serum albumin and [7-3H] -DEA. The method is highly sensitive (0.039 μmol/l), specific, reliable, and easily reproducible. This method was used to measure plasma DEA-S concentrations in healthy subjects of various ages, in women in different phases of the cycle, after dexamethasone load, and in patients with the Stein-Loewenthal's syndrome and the adrenogenital syndrome. It is recommended for the diagnosis of retinopathies connected with adrenal and gonadal androgenic dysfunction.

About the Author

N D Goncharova
НИИ экспериментальной патологии и терапии, Сухуми


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Review

For citations:


Goncharova N.D. DIRECT RADIOIMMUNOASSAY OF BLOOD PLASMA DEHYDROEPIANDROSTERONE SULFATE AND ASSESSMENT OF THE ADRENOCORTICAL ANDROGENIC FUNCTION. Problems of Endocrinology. 1993;39(3):26-29. https://doi.org/10.14341/probl199339326-29

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