Features of aging replicative and biochemical aspects among females with non-iatrogenic hypergonadotropic hypogonadism

BACKGROUND: Estrogenic deficiency is the basic condition of human ageing that leads to hypergonadotropic hypogonadism. The existence of correlation between hypergonadotropic hypogonadism, replicative (leukocyte telomere length) and biochemical data is widely supposed among females with physiological (menopausal) and pathological (primary ovarian insufficiency) estrogenic deficiency is not unreasonable.

AIM: To evaluate features of replicative (telomere length) and biochemical (metabolic syndrome) ageing markers among females with physiological (menopausal) and pathological (primary ovarian insufficiency) estrogenic deficiency.

MATERIALS AND METHODS: Research has been provided in collaboration between Endocrinology Research Centre of the Russian Ministry of Health and Lomonosov Moscow State University Medical Research and Educational Centre in the period since 10.01.2021 until 01.08.2022.

110 females (20-75y.o.) have participated in the present research.

Group 1: 26 females receiving menopausal hormonal therapy (MHT) ≥ 5 years with 0,5; 1; 2 mg estrogenic component.

Group 2: 27 females in physiological menopause without MHT

Group 3: 33 females with primary ovarian insufficiency and receiving sex-steroid replacement therapy.

Group 4: 24 healthy reproductive age females without sex-steroid replacement therapy.

Patients have undergone laboratory genetic (leucocyte telomere length), biochemical analyses.

DNA extraction — with Qiagen DNA blood mini kit (Germany). Biological material was cito conserved with Ficoll solution. Leukocyte telomere length — with real-time polymerase chain reaction PCR (Flow-fish).

Soft program IBM SPSS Statistics (version 26,0 for Windows) has been used for statical analysis.

RESULTS.

1.Menopausal females receiving MHT were inclined to highest HDL-P levels (p<0,006).

2.Females with primary ovarian insufficiency were inclined to relatively highest serum creatinine level (p<0,001).

3.Reproductive age females had relatively highest telomere length (p<0,001).

4.FSH level correlates negatively and moderately (ρ= - 0,434) leukocyte telomere length (р<0,001) among females.

CONCLUSION: Females with premature ovarian insufficiency are most sensible to ageing due to features of replicative and biochemical markers.

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