The activity of lysosomal serum enzymes and blood leukocytes in patients with type 1 diabetes
https://doi.org/10.14341/probl20004653-6
Abstract
Activities of 3 lysosomal enzymes in blood serum and leukocytes were studied in 115 with type 1 diabetes with and without vascular complications, different glycaemic and lipid peroxidation (LPO) status. Serum activities of N-acetylglucosaminidase and p-galactosidase were increased by 1.7 times and of acid RNAse by 1.6 times in patients with decompensated diabetes vs. the control. Shifts in enzymatic activities did not disappear with compensation of disease. Increased activities of lysosomal enzymes were observed in the patients with first diagnosed diabetes mellitus. The highest levels were observed in patients with pronounced vascular complications. Deficient activities of N-acetylglucosaminidase and p-galactosidase were detected in the blood leukocytes of these patients. Correlations between changes in the serum and leukocyte activities of lysosomal enzymes and accumulation of lipid peroxides (dienic conjugates and malonic dialdehyde) in erythrocyte membranes were disclosed. These data indicate alteration of the cellular lysosomal system in diabetics, contribution of LPO reactions to these shifts, and their relationships with development of microangiopathies.
About the Authors
I. A. Bondar'Novosibirsk Medical University
Russian Federation
A. B. Pupyshev
Novosibirsk Medical University
Russian Federation
V. V. Klimontov
Novosibirsk Medical University
Russian Federation
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Review
For citations:
Bondar' I.A., Pupyshev A.B., Klimontov V.V. The activity of lysosomal serum enzymes and blood leukocytes in patients with type 1 diabetes. Problems of Endocrinology. 2000;46(5):3-6. (In Russ.) https://doi.org/10.14341/probl20004653-6
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