Use of the ultrasensitive technique to determine biologically active salivary free Cortisol for evaluating the glucocorticoid function of the adrenal cortex
https://doi.org/10.14341/probl200854327-35
Abstract
Comparative studies were conducted to define the informative value of the noninvasive technique for determining free Cortisol in saliva and daily urine, by applying ultrasensitive immunoassay technologies, and for evaluating total Cortisol in blood from healthy individuals and patients with Cushing's syndrome. The studies were made during functional tests and glucocorticoid replacement therapy before and after treatment. To evaluate the pattern of diurnal rhythm, the level of total Cortisol in the blood and that of its free form in the saliva were simultaneously measured in the morning, in the afternoon, and in the evening. The normal daily salivary free Cortisol values were established in healthy individuals in the morning (6.8-25.9 nmol/l) and in the evening (0.6-3.3 nmol/l). There is evidence that salivary free Cortisol is highly sensitive in the assessing functional tests with adrenocorticotropic hormone and dexamethasone. The noninvasiveness, accessibility, and easiness-to-use of obtaining salivary samples in combination with the high analytical sensitivity and specificity of the technique in determining the free biologically active fraction of Cortisol make it promising in evaluating adrenal hormonal function.
About the Authors
N. P. GoncharovFederal State Institution Endocrinological Research Center of ''Rosmedtehnologii''
Russian Federation
G. V. Kaimia
Federal State Institution Endocrinological Research Center of ''Rosmedtehnologii''
Russian Federation
Ye. I. Marova
Federal State Institution Endocrinological Research Center of ''Rosmedtehnologii''
Russian Federation
G. S. Kolesnikova
Federal State Institution Endocrinological Research Center of ''Rosmedtehnologii''
Russian Federation
S. D. Arapova
Federal State Institution Endocrinological Research Center of ''Rosmedtehnologii''
Russian Federation
L. Ya. Rozhinskaya
Federal State Institution Endocrinological Research Center of ''Rosmedtehnologii''
Russian Federation
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Review
For citations:
Goncharov N.P., Kaimia G.V., Marova Ye.I., Kolesnikova G.S., Arapova S.D., Rozhinskaya L.Ya. Use of the ultrasensitive technique to determine biologically active salivary free Cortisol for evaluating the glucocorticoid function of the adrenal cortex. Problems of Endocrinology. 2008;54(3):27-35. (In Russ.) https://doi.org/10.14341/probl200854327-35
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