On the interaction of cytosolic complexes of the glucocorticoid receptor and synthetic glucocorticoid responsive element
https://doi.org/10.14341/probl199844341-44
Abstract
The constant of affinity between glucocorticoid receptor (GR) complex isolated together with triamcinolone and a synthetic oligonucleotide glucocorticoid-responsive element (GRE) corresponding to the GRE-1 sequence of the rat thyrosine aminotransferase gene promoter is assessed. Singlestranded oligonucleotides with the straight sequence (Kd 0.2 ± 0.006 pM) possess the highest affinity for GR. The affinity of single-stranded oligonucleotides with antisense sequence is far lower (Kd 1.5 ± 0.2 pM), but is superior to that of double-stranded oligo GRE (Kd 12.0+ 1.1 pM). The results suggest that the complexes are formed mainly with the sense strand of DNA; on the other hand, it is probable that the reaction of glucocorticoid receptor with GRE promotes conformational changes of DNA in this gene region.
References
1. Beato М., Bruggemeier U., Chalepakis G. et al. // The Hormonal Control Regulation of Gene Transcription. — Amsterdam, 1991. - P. 117-128.
2. Ben-Or S., Okret S. // Mol. Cell Biol. - 1993. - Vol. 13. - P. 331-340.
3. Cairns C. Mechanisms and Role of DNA-Binding in Steroid Hormone Receptor Action: Ph. D. Thesis. Biology. Repro Print. AB. — Stockholm, 1993.
4. Dahlman-Wright K., Wright A. P. H., Gustafsson J.-A. // Biochemistry. — 1992. — Vol. 31. — P. 9040—9044.
5. Eriksson P., Wrange O. // J. biol. Chem. — 1990. — Vol. 265. - P. 3535-3542.
6. Herd T., Kllenbach E., Boelens R. et al. // Science. — 1990. — Vol. 249. - P. 157-160.
7. McEwan I. J., Wright A. P. H., Dahlman-Wright K. et al. // Mol. Cell Biol. - 1993. - Vol. 13. - P. 399-407.
8. Strahle U., Schmid W., Schutz G. // EMBO J. - 1988. - Vol. 7. - P. 3389-3395.
9. Suzuki T., Matsuno K., Takiya S. et al. // J. biol. Chem. — 1991. _ Vol. 266. - P. 16935—16941.
10. Zilliacus J., Wright A. P. H., Norinder U. et al. // J. biol. - Chem. - 1992. - Vol. 267. - P. 24941-24947.
Review
For citations:
Solenov E.I. On the interaction of cytosolic complexes of the glucocorticoid receptor and synthetic glucocorticoid responsive element. Problems of Endocrinology. 1998;44(3):41-44. (In Russ.) https://doi.org/10.14341/probl199844341-44
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