Thyroglobulin gene expression in congenital goiter
https://doi.org/10.14341/probl11823
Abstract
A polyA-containing thyroglobulin mRNA (mRNAptf) was isolated from human thyroid in health and congenital goiter. A singlestranded complementary DNA (cDNA) was synthesized on a polyA-containing mRNATl[ matrix by reverse transcription in the presence of RNAse inhibitor from human placenta; this cDNA was used in hybridization experiments for measurements of mRNA. The amount of mRNApH in the presence of TH deficiency was normal in the examined goiter case, indicating an impaired TH gene expression at the translation level.
About the Authors
D. A. KadyrovInstitute of Biochemistry, Academy of Sciences of the Republic of Uzbekistan
Uzbekistan
B. A. Atakhanova
Institute of Biochemistry, Academy of Sciences of the Republic of Uzbekistan
Uzbekistan
Y. Kh. Turakulov
Institute of Biochemistry, Academy of Sciences of the Republic of Uzbekistan
Uzbekistan
References
1. Атаханова Б. А., Кадырова Д. А., Туракулов Я. X., Шипицына Г. И. // Биохимия. — 1989. — Т. 54, № 1. — С. 81—87.
2. Гайцхоки В. С. // Биополимеры и клетка. — 1990. — Т. 6, № 11. С. 31-46.
3. Кадырова Д. А., Атаханова Б. А., Туракулов Я. X. и др. // Биохимия. — 1979. — Т. 44, № 2. — С. 259—263.
4. Кадырова Д. А., Атаханова Б. А., Туракулов Я. X., Шипи цына Г. И. I/ Молекул, биол. — 1989. — Т. 23, № 4. — С. 1101-1106.
5. Лейтин В. Л., Лерман М. И. // Биохимия. — 1969. — Т. 34. — С. 839-850.
6. Туракулов Я. X., Бабаев Т. А., Сайтов Т. С. Йодпротеины щитовидной железы. — Ташкент, 1974.
7. Туракулов Я. X., Атаханова Б. А., Кадырова Д. А. // Пробл. эндокринол. — 1983. — Т. 29, № 5. — С. 38—41.
8. Kafatos F. С., James С. Н. // Nucl. Acids Res. — 1979. — Vol. 7. P. 1541-1551.
9. De Martinoff R. S. E., Vassart G. // Biochem. biophys. Res. Commun. — 1970. — Vol. 93. — P. 645—654.
10. Richets M. H., Pohl V., De Martinoff C. D. et al. // Eur. molec. Biol. Organ. 1985. Vol. 44, N 3. P. 731-737.
11. Rigby R. W. J., Dickman M., Ponder G. // J. molec. Biol. — 1977. Vol. 113. P. 237-251.
12. Sterk A., van Dijk J., Veenber J. M. et al. // Endocrinology. — 1989. Vol. 124, N 1. P. 477-483.
Review
For citations:
Kadyrov D.A., Atakhanova B.A., Turakulov Y.Kh. Thyroglobulin gene expression in congenital goiter. Problems of Endocrinology. 1999;45(6):34-36. (In Russ.) https://doi.org/10.14341/probl11823
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