The effect of thyroxine isomers on the processes of free radical oxidation in the subcellular fractions of the rat cerebral cortex
https://doi.org/10.14341/probl11862
Abstract
Effective concentrations for D and L thyroxin isomers were determined by the chemiluminescent (CL) method and their effects on free radical oxidation in the mitochondrial and synaptosomal fraction of adult rat cerebral cortex were studied in vitro. A OA DT4 in a model system with riboflavin was 2.2 times higher than L-T4. Effective concentrations for both thyroxin forms were 1$q= = 7.43 x 10~5+/-0.71 M for D-T4 and 15q=15.47 x 10~5+/1.23 M for L-T4. Thyroxin effect on membranous fraction of the brain cortex was studied in vitro using luminol-dependent peroxide CL. In normal concentrations (1 x 108 M) both hormone forms exerted equally intensive antioxidant effect which was more pronounced in the mitochondrial fraction, where CL decreased by 69 and 66%, while in the synaptosomal fraction it decreased only by 45 and 46%. Since D form possesses no hormonal activity, this effect may be due to phenol origin of thyroxin.
About the Authors
O. V. GalkinaInstitute of Physiology A. A. Ukhtomsky; St. Petersburg State University; Institute of Obstetrics and Gynecology. D.O. Ott RAMS
Russian Federation
V. M. Prokopenko
Institute of Physiology A. A. Ukhtomsky; St. Petersburg State University; Institute of Obstetrics and Gynecology. D.O. Ott RAMS
Russian Federation
F. E. Putilina
Institute of Physiology A. A. Ukhtomsky; St. Petersburg State University; Institute of Obstetrics and Gynecology. D.O. Ott RAMS
Russian Federation
N. D. Yeshchenko
Institute of Physiology A. A. Ukhtomsky; St. Petersburg State University; Institute of Obstetrics and Gynecology. D.O. Ott RAMS
Russian Federation
A. V. Arutyunyan
Institute of Physiology A. A. Ukhtomsky; St. Petersburg State University; Institute of Obstetrics and Gynecology. D.O. Ott RAMS
Russian Federation
References
1. Антипенко Е. Н., Антипенко А. Е., Кавешникова И. В. и др. // Успехи совр. биол. — 1994. — Т. 114. Вып. 5. — С. 558—572.
2. Бурмистров С. О.. Опарина Т. И., Прокопенко В. М.. Арутюнян А. В. // Клин. лаб. диагн. — 1997. — № 11. — С. 14-17.
3. Владимиров Ю. А. Сверхслабые свечения при биохимических реакциях. — М., 1966.
4. Владимиров Ю. А., Гусаков В. М., Федоров В. К. и др. // Бюл. экспер. биол. — 1977. — Т. 83, № 5. — С. 558—560.
5. Галкина О. В. Вестн. СПбГУ. — 1995. — Сер. 3. — Вып. 2. № 10. С. 63-67.
6. Прокопенко В. М., Арутюнян А. В., Фролова Е. В. и др. // Бюл. экспер. биол. — 1997. — Т. 124, № 12. — С. 632—634
7. Сергеев В. П. Стероидные гормоны. — М., 1984.
8. Шерстнев М. П. // Вопр. хемилюминесценции. — 1991. — Т. 1, № 2. С. 13-15.
9. Aoyagi К., Takeshige К., Minakami S. // Biochim. Biophys. Acta. 1991. Vol. 1093, N 2/3. P 223-228.
10. Bastiaanse E., Hold K.. Van der Laarse A. // Cardiovasc. Res. — Vol. 33, N 2. P. 272-283.
11. Hajos F. // Brain Res. 1975. Vol. 93, N3.-P. 485-489.
12. Hulbert A. J. /I J. Theoret. Biol. — 1978. — Vol. 73. N I. — P 81-100.
13. Morini P., Casalino E., Sblano C., Landriscina C. // Int. J. Biochem. 1991. Vol. 23, N 10. P. 1025-1030.
14. Venditti P., Balestrieri M., Di Meo S., De Leo T. // J. Endocrinol. 1997. Vol. 155, N 1. P. 151-157.
15. Wynn J. // Arch. Biochem. — 1968. — Vol. 126, N 3. — P. 880-891.
Review
For citations:
Galkina O.V., Prokopenko V.M., Putilina F.E., Yeshchenko N.D., Arutyunyan A.V. The effect of thyroxine isomers on the processes of free radical oxidation in the subcellular fractions of the rat cerebral cortex. Problems of Endocrinology. 2000;46(4):32-34. (In Russ.) https://doi.org/10.14341/probl11862
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