Urinary hydroxyproline excretion and glomerular location of collagen III, IV, and VI in patients with type 1 diabetes mellitus concurrent with nephropathy
https://doi.org/10.14341/probl20055146-10
Abstract
The study was undertaken to investigate the metabolism of collagen and the accumulation of collagens III, IV, and VI in the glomeruli in patients with type 1 diabetes mellitus (DM) and early-stage nephropathies. The urinary excretion of peptide-bound and free hydroxyproline was determined in 57 patients with DM and 15 healthy individuals. Immunohistochemical investigations of their renal biopsy specimens were performed in 17 patients, by using monoclonal antibodies to collagens III, IV, and VI. The kidneys from 70 healthy individuals who had died due accidents served as a control. A significantly increased urinary excretion of peptide-bound hydroxyproline was found in patients with DM and micro- and macroalbuminuria. There was an excessive glomerular accumulation of collagens IV and VI in 8 and 7 patients, respectively. Interstitial collagen HI was detected in the glomeruli of 9 patients while it was absent in the controls. The findings show the high rates of collagen metabolism and collagens III, IV, and VI accumulation in the glomeruli of patients with type 1 DM and early-stage diabetic nephropathy.
About the Authors
I. A. BondarNovosibirsk State Medical Academy
Russian Federation
V. V. Klimontov
Novosibirsk State Medical Academy
Russian Federation
A. P. Nadeev
Novosibirsk State Medical Academy
Russian Federation
L. B. Kim
Research Institute of Experimental & Clinical Medicine
Russian Federation
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Review
For citations:
Bondar I.A., Klimontov V.V., Nadeev A.P., Kim L.B. Urinary hydroxyproline excretion and glomerular location of collagen III, IV, and VI in patients with type 1 diabetes mellitus concurrent with nephropathy. Problems of Endocrinology. 2005;51(4):6-10. (In Russ.) https://doi.org/10.14341/probl20055146-10
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