Androgen deficiency and problems of its diagnosis by current nonisotopic testosterone assays
https://doi.org/10.14341/probl200854530-39
Abstract
Current hormonal immunoassays of blood total and free testosterone fail to provide an adequate diagnostic accuracy in most cases. The official statement mode by the International Association of Endocrinologists in 2007 on the inadmissibility of using direct im-munoassays, including all analyzers, to measure testosterone in women and children Is a result of an analysis of this problem. Tandem mass-spectrometry (TD-MS) that guarantees accuracy, specificity, and a reduction in the effect of a matrix on measurement of tes-tosterone or any other steroid should be ideally used as a reference technique. The paper reviews preanalytical and analytical factors influencing the diagnostic accuracy of evaluation of the androgen status in males, females, and children. A specific and highly sensitive (8.7 pmol/l) technology of luminescence immunoassay of salivary free testosterone, which had been introduced into diagnostic practice, considerably improved the diagnostic accuracy of determination of testosterone in both males and females and children. The paper also presents the authors' results of determination of free testosterone in salivary microvolumes in healthy and androgen-deficiency males, by taking into account physiological diurnal variations. The values of salivary free testosterone in combination with clinical symptoms are shown to be used as an objective and adequate hormonal marker of diagnosis of different forms of male androgen deficiency.
About the Authors
N. P. Goncharov
Endocrinology Research Center of Russian Medical Technologies
Russian Federation
Russian Federation
G. V. Katsia
Endocrinology Research Center of Russian Medical Technologies
Russian Federation
Russian Federation
A. D. Dobracheva
Endocrinology Research Center of Russian Medical Technologies
Russian Federation
Russian Federation
N. M. Malysheva
Endocrinology Research Center of Russian Medical Technologies
Russian Federation
Russian Federation
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Review
For citations:
Goncharov N.P., Katsia G.V., Dobracheva A.D., Malysheva N.M. Androgen deficiency and problems of its diagnosis by current nonisotopic testosterone assays. Problems of Endocrinology. 2008;54(5):30-39. (In Russ.) https://doi.org/10.14341/probl200854530-39
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