A modified radioimmunoassay of aldosterone

Abstract

A method of radioimmunoassay of aldosterone in the blood of humans and monkeys has been developed. Antiserum was prepared on rabbits against aldosterone-3-(0-carboxymethyloxime) conjugate with bovine serum albumin. The specificity of antiserum was tested in cross-reactions with 32 related steroids. High specificity of the antiserum in this method helped measure aldosterone levels without preliminary chromatographic isolation and purification of this compound. The sensitivity of the new method is 12.5 pg per 0.1 ml. Reproducibility of the method between different series of measurements was no more than 15%, within the same series below 12%. The method was used to assess the mineralocorticoid function of the adrenal cortex of man and monkeys.