NPY-Synthesizing cells of Langerhans’ islets in normal and diabetic rats after injection of synthetic neuropeptide Y
https://doi.org/10.14341/probl11530
Abstract
The purpose of this study was identification and evaluation of NPY-synthesizing cells of Langerhans ’ islets in Wistar rats in health, streptosotocin diabetes of different duration, and during chronic treatment of diabetic animals by synthetic NPY. NPY- and insulin-producing cells in the islets were identified by indirect immunofluorescence using Peninsula Laboratories Inc. kits (USA) and their status was analyzed by VIDAS-386 image processing (Kontron Elektronic, Germany). NPY-immunoreactive cells of different shape and size were detected in the islets of normal animals. Three-week development of diabetes led to more than twofold increase in their count in comparison with the control, indicating activation of the peptide synthesis, which normalized by day 35 of disease. Injection of synthetic peptide from day 26 to day 35 of diabetes largely restored the count and function of p-cells, prevented the inhibition of NPYsynthesis in the islets, and stimulated its secretion.
About the Authors
A. V. TrailinZaporizhzhya State Medical University
Ukraine
Yu. M. Kolesnik
Zaporizhzhya State Medical University
Ukraine
M. A. Orlovsky
Zaporizhzhya State Medical University
Ukraine
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Review
For citations:
Trailin A.V., Kolesnik Yu.M., Orlovsky M.A. NPY-Synthesizing cells of Langerhans’ islets in normal and diabetic rats after injection of synthetic neuropeptide Y. Problems of Endocrinology. 2001;47(3):36-41. (In Russ.) https://doi.org/10.14341/probl11530
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