Polymorphism of microsatellite markers of aldose reductase and catalase genes and a genetic predisposition to nephropathy in insulin-dependent diabetes mellitus

Polymorphism of dinucleotide microsatellite in the aldose reductase (AR2) gene and two polymorphic microsatellite markers (DI 1S907 and DI IS2008) near catalase (CAT) gene were studied in insulin-dependent diabetics with and without diabetic nephropathy (DN). We formed the groups of patients to compare using the "extremephenotype"approach. Twenty-seven proteinuric patients with relatively short diabetes duration (<15 years) and 41 patients with a longer diabetes duration (>20years) without signs of DNserved as the "case"(DN+) and "control"(DN-) groups, respectively. Seven alleles of the AR2 gene ranging from 132 to 144 base pairs (bp) long were detected. For DI 1S907 and DI 1S2008, seven and eight alleles from 161 to 173 and from 120 to 148 bp long were found, respectively. No significant differences in the distribution of alleles and genotypes of AR2 and DI IS2008 loci in the DN+ and DN— groups were detected by Fisher's test. For D11S907, a significant increase in the frequency of both 15 (16.7% vs. 6.1%), p<0.05) and 18 (29.6%) vs. 15.9%, p<0.05) alleles was revealed in DNpatients in comparison with the controls, while the 17 allele of D11S907 was significantly rarer (9.3% vs. 30.5%, p<0.005) in the "nephropaths" than in diabetics without DN. Hence, the polymorphism of the AR2 gene is not associated with DN in IDDM, while a chromosome region near the CA T gene is likely to be linked with the disorder.

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