Kinetic parameters of GABA receptor binding by rat adenohypophysis membranes under conditions of modeling various levels of corticosteroids and ACGT in the body
https://doi.org/10.14341/probl11949
Abstract
Kinetic parameters of l4C-GABA specific binding by rat adenohypophyseal plasma membranes were studied in experiments on modelling various corticosteroid and ACTH levels in animal body. A single hydrocortisone injection did not change Ka for high- and low-affinity GABA receptors, the number of the former (Bmax) increasing in this case. Repeated hydrocortisone injections were associated with Ko reduction for high-affinity GABA receptors and a noticeable increase of Kn for low-affinity receptors, with their number reducing. ACTH injection did not change the kinetic parameters of GABA binding with receptors. The number of high-affinity GABA receptors and their affinity reduced after removal of adrenals whereas the number of low-affinity receptors in this case was increasing. A single hydrocortisone injection to adrenalectomized rats had a normalizing effect on adenohypophyseal GABA receptors. Analysis of the results and changes in blood hormonal levels indicated that affinity changes in high-affinity receptors and changed number of low-affinity adenohypophyseal GABA receptors correlated with changes in ACTH and hydrocortisone changes.
About the Authors
T. M. MishuninaKomissarenko Research Institute of Endocrinology and Metabolism of the Ministry of Health of Ukraine
Ukraine
V. Ya. Kononenko
Komissarenko Research Institute of Endocrinology and Metabolism of the Ministry of Health of Ukraine
Ukraine
References
1. Кириаков Г. В., Никурадзе В. О., Рожанец В. В. Эффективность связывания — новый показатель для сравнения состояния рецепторов.— М., 1985. (Рукопись деп. в ВИНИТИ, № 6182—85 Деп.)
2. Лакин Г. Ф. Биометрия.— М., 1980,
3. Машилов К. В., Кириаков Г. В., Малин К. М. и др. // Бюл. экспер. биол.— 1987.— № 4.— С. 506—508.
4. Мишунина Т. М., Кононенко В. Я- // Укр. биохим. журн,— 1990,— Т. 62, № 6,— С. 71—79.
5. Мишунина Т. М„ Кононенко В. Я. // Докл. АН УССР.— 1990,— № 10,— С. 63—66.
6. Мишунина Т. М., Кононенко В. Я- // Бюл. экспер. биол.— 1991,— № 1,— С. 42—43.
7. Сергеев П. В., Шимановский Н. Л. Рецепторы физиологически активных веществ,— М., 1987.
8. Acs Z.. Stark Е. // J. Endocr.— 1978,— Vol. 77, N 1,— Р. 137—141.
9. Demeneix В., Feliz Р., Loeffler J. // GABAergic Mechanism in the Mammalian Periphery / Eds S. Erdo,N. Boery.— New York, 1986,— P. 261—289.
10. Kendal D., McEwen B., Enna S. // Brain Res.— 1982.— Vol. 236, N 2,— P. 365—374.
11. Majewska M., Bisserbe J.-C., Eskay R. // Ibid.— 1985.— Vol. 339, N 1,— P. 178—182.
12. Supaviali P., Cortes R., Palacios J. et al. // Modulation of Central and Peripheral Transmitter Function / Eds G. Biggio et al.— New York, 1986.— P. 453—457.
13. Sutanto W., Handelman G., De Kloet E. // Neuroendocr. Lett.— 1988,— Vol. 10, N 4,— P. 280
Review
For citations:
Mishunina T.M., Kononenko V.Ya. Kinetic parameters of GABA receptor binding by rat adenohypophysis membranes under conditions of modeling various levels of corticosteroids and ACGT in the body. Problems of Endocrinology. 1993;39(6):43-46. (In Russ.) https://doi.org/10.14341/probl11949
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