Immunological approaches to the prediction of insulin-dependent diabetes mellitus
https://doi.org/10.14341/probl19994563-6
Abstract
A total of 150 patients with carbohydrate metabolism disorders were examined: 58 with insulin-dependent diabetes mellitus (IDDM), 14 with impaired tolerance of easily assimilable carbohydrates, 67 subjects at risk of these conditions (sibs), 11 patients with non-insulin-dependent diabetes mellitus (NIDDM), and 50 donors. Autoantibodies to pancreatic islet cells (ISA) were tested by two methods: indirect immunofluorescence with cryosections of human pancreas and enzyme immunoassay with the Isletest kits (Biomerica). In IDDM patients with the disease duration of up to 2 years, ISA were detected in 66.7% cases on cryosections of human pancreas and in 50% cases by the Isletest. There was a general tendency to a lower rate of ISA detection with a longer duration of the disease. The results of the two methods did not agree. Autoantibodies to insulin (IAA) were detected in 37.5%) of these patients, which indicates the need in a comprehensive approach to immunodiagnosis of the preclinical stages of IDDM.
About the Authors
Ya. S. ZverevaState Scientific Center "Institute of Immunology" of the Ministry of Health of the Russian Federation, Moscow Medical Academy I. M. Sechenova
Russian Federation
I. V. Dubinkin
State Scientific Center "Institute of Immunology" of the Ministry of Health of the Russian Federation, Moscow Medical Academy I. M. Sechenova
Russian Federation
A. V. Zilov
State Scientific Center "Institute of Immunology" of the Ministry of Health of the Russian Federation, Moscow Medical Academy I. M. Sechenova
Russian Federation
N. Taklas
State Scientific Center "Institute of Immunology" of the Ministry of Health of the Russian Federation, Moscow Medical Academy I. M. Sechenova
Russian Federation
L. P. Alekseev
State Scientific Center "Institute of Immunology" of the Ministry of Health of the Russian Federation, Moscow Medical Academy I. M. Sechenova
Russian Federation
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Review
For citations:
Zvereva Ya.S., Dubinkin I.V., Zilov A.V., Taklas N., Alekseev L.P. Immunological approaches to the prediction of insulin-dependent diabetes mellitus. Problems of Endocrinology. 1999;45(6):3-6. (In Russ.) https://doi.org/10.14341/probl19994563-6
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