The ultrastructure and function of lymphocytes in children with first detected untreated type 1 diabetes mellitus
https://doi.org/10.14341/probl20055158-13
Abstract
Flow cytometry (FACS-analysis), light and electron microscopy, and cytotoxic test were used to study the leukocytic composition, content, ultrastructure, and function of different lymphocytic populations (CD3+, CD4+, CD8+, CD20+, and CD56+ cells) in the blood of 90 children aged 8 to 15 years who had untreated new-onset type 1 diabetes mellitus. A control group consisted of 45 apparently healthy normoglycemic children. The sick children were observed to have a slight, but statistically significant reduction in the relative and absolute blood content of CD3+, CD4+, and CD56+ cells, CD4/CD8 index and particularly large granule-containing lymphocytes (the morpho-logical homologue of natural Idller (NK) cells). Ultrastructurally, CD20+4 cells (lymphocytes containing Gall's bodies) showed the signs of a high functional activity while NK cells (large granule-containing lymphocytes) exhibited a low one, which was confirmed by the decreased cytotoxic activity of these cells determined in vitro. Three-month insulin therapy leading to the restoration of the blood content of glucose and glycosylated hemoglobin fails to normalize the detected changes in the parameters of T- and NK-cell immunity.
About the Authors
K. P. ZakV.P. Komisarenko Institute of Endocrinology and Metabolism
Ukraine
M. A. Gruzov
V.P. Komisarenko Institute of Endocrinology and Metabolism
Ukraine
V. V. Afanasyeva
V.P. Komisarenko Institute of Endocrinology and Metabolism
Ukraine
T. N. Malinovskaya
V.P. Komisarenko Institute of Endocrinology and Metabolism
Russian Federation
V. V. Popova
V.P. Komisarenko Institute of Endocrinology and Metabolism
Ukraine
A. V. Kulikovskaya
V.P. Komisarenko Institute of Endocrinology and Metabolism
Ukraine
T. A. Semionova
V.P. Komisarenko Institute of Endocrinology and Metabolism
Ukraine
T. F. Zakharchenko
V.P. Komisarenko Institute of Endocrinology and Metabolism
Russian Federation
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Review
For citations:
Zak K.P., Gruzov M.A., Afanasyeva V.V., Malinovskaya T.N., Popova V.V., Kulikovskaya A.V., Semionova T.A., Zakharchenko T.F. The ultrastructure and function of lymphocytes in children with first detected untreated type 1 diabetes mellitus. Problems of Endocrinology. 2005;51(5):8-13. (In Russ.) https://doi.org/10.14341/probl20055158-13
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