A fragment of apolipoprotein B with insulin-like immunoreactivity

Abstract

The purpose of this study was immunochemical analysis of a fragment of apolipoprotein В with insulin-like immunoreactivity. We denoted it as peptide B. The peptide isolated by electrophoretic elution was used to obtain specific antibodies. Antibodies to peptide В reacted with apolipoprotein B-100, whole serum, serum after precipitation of $\beta$-lipoproteins, and with supernatant after removal of all lipoproteins by ultracentrifugation. Enzyme immunoassay was optimized for evaluation of serum peptide В after precipitation of $\beta$-lipoproteins. Serum concentrations of peptide В were increased in patients with type 2 diabetes mellitus in comparison with donors. The content of peptide В increased with increase of body weight index and disease duration.